Prokaryotic expression of DFP1 and DFP2 in Dermatophagoides farinae and their responses to temperature stress

• Published in: Gene Vol. 927; p. 148661
• Main Authors: Wanyu, Zhang, Dongling, Niu, Yae, Zhao, Lianying, Jiao, Chenglin, Guan, Rong, Chai, Li, Hu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2024
• Subjects: Bacterial growth curves; bioinformatics; cold stress; Dermatophagoides farinae; Dermatophagoides farinae proteins; Escherichia coli; genes; heat; Prokaryotic expression; protein content; sequence analysis; stress response; temperature; Temperature stress; Temperature stress; nt; D. farinae; dNTP; kDa; Mw; HSPs; CDS; RNA-seq; OD; kb; E. coli; Bacterial growth curves; Prokaryotic expression; SDS; Amp; CAI; PAGE; Dermatophagoides farinae proteins; ORFs; PMSF; LB; IPTG; DFPs; DFP2; DFP1

[Display omitted] •DFP1 &DFP2 are highly expressed in D. farinae but their functions are unknown.•DFP1 & DFP2 were expressed in E. coli Rosetta-gami 2 & BL21, respectively.•After temperature stress, recombinant strains grow better than controls.•DFP1 recombinant strains grow better under...