Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase

• Published in: Biosensors & bioelectronics Vol. 177; p. 112984
• Main Authors: Hatada, Mika, Saito, Satomi, Yonehara, Satoshi, Tsugawa, Wakako, Asano, Ryutaro, Ikebukuro, Kazunori, Sode, Koji
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.04.2021
• Subjects: 2.5th generation principle; Ascomycota; Biosensing Techniques; biosensors; diabetes; electrochemistry; electron transfer; Electrons; Flow Injection Analysis; Fructosyl peptide oxidase; Glycated Hemoglobin A - analysis; Glycated protein; glycemic control; glycohemoglobin; Haemoglobin A1c; hemoglobin; Humans; mutants; oxidoreductases; Parastagonospora nodorum; Peptides; proteinases; Quasi-direct electron transfer; valine; Glycated protein; 2.5th generation principle; Flow injection analysis; Fructosyl peptide oxidase; Haemoglobin A1c; Quasi-direct electron transfer

Haemoglobin A1c (hemoglobin A1c, HbA1c) is an important long-term glycemic control marker for diabetes. The aim of this study was to develop an enzyme flow injection analysis (FIA) system using engineered fructosyl peptide oxidase (FPOx) based on 2.5th generation principle for an HbA1c automated ana...