Influence of hypoxia and hypoxia/hypercapnia upon brain and blood peroxidative and glutathione status in normal weight and growth-restricted newborn piglets

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie Vol. 50; no. 4-6; pp. 402 - 410
• Main Authors: Barth, A., Bauer, R., Gedrange, T., Walter, B., Klinger, W., Zwiener, U.
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.09.1998; Elsevier
• Subjects: Acid-Base Equilibrium; Animals; Animals, Newborn; Biological and medical sciences; Blood Gas Analysis; Blood Pressure; Blood, peroxidative status; Body Weight; Brain - metabolism; Brain - physiopathology; Brain, hypoxia, hypercapnia; Brain, peroxidative status; Delivery. Postpartum. Lactation; Fetal Growth Retardation - metabolism; Fetal Growth Retardation - physiopathology; Glutathione; Glutathione - metabolism; Gynecology. Andrology. Obstetrics; Hypercapnia; Hypercapnia - metabolism; Hypercapnia - physiopathology; Hypoxia; Hypoxia, Brain - metabolism; Hypoxia, Brain - physiopathology; Lipid Peroxidation; Lipid Peroxides - metabolism; Medical sciences; Obstetrical techniques; Oxygen free radicals; Peroxidative status, blood, brain; Peroxides - metabolism; Radicals, oxygen free; Reactive Oxygen Species - metabolism; Swine; Thiobarbituric Acid Reactive Substances - metabolism; Hypercapnia; Radicals, oxygen free; Brain, hypoxia, hypercapnia; Oxygen free radicals; Lipid peroxidation; Hypoxia; Peroxidative status, blood, brain; Blood, peroxidative status; Brain, peroxidative status; Glutathione; Premature; Oxidative stress; Pregnancy disorders; Pathogenesis; Carbon dioxide; Central nervous system; Oxidant; Growth retardation; Blood; Very low birthweight; Ungulata; Peroxidation; Oxygen; Respiratory disease; In utero; Pig; Newborn diseases; Vertebrata; Experimental disease; Mammalia; Newborn animal; Animal; Artiodactyla; Brain (vertebrata)
• DOI: 10.1016/S0940-2993(98)80026-2
• ISSN: 0940-2993

Glutathione (reduced (GSH) and oxidized (GSSG)), lipid peroxidation products (TBAR) and in vitro production of reactive oxygen species (ROS, by means of stimulated lipid peroxidation, H2O2 formation and amplified chemiluminescence (CL) in 9000 xg brain supernatants) were studied in the cerebellum (C) and temporoparietal area (TP) of the brain of normal weight (NW) and spontaneously intra-uterine growth-restricted newborn piglets (IUGR) after 1 hour hypoxia (fractional inspired oxygen concentration (FiO2) 8%), and in combination with 10% CO2, followed by 3 hours recovery (FiO2 30%). The strong GSH depletion accompanied by an increased concentration of GSSG and TBAR, more distinct in IUGR, is the most important result in the brain after hypoxia and reoxygenation. Hypercapnia-related acidosis seems to protect the brain of IUGR from hypoxia/reoxygenation induced injury by reducing GSH depletion as well as GSSG and TBAR increases. But stimulated lipid peroxidation and H2O2 formation in 9000 ×g supernatants of C and TP were found to be higher in acidosis and hypercapnia. Decreased or unchanged amplified CL, demonstrating lower in vitro production of ROS, cannot explain the GSH depletion after hypoxia and reoxygenation. The scarce changes in erythrocyte GSH and GSSG as well as plasma TBAR concentrations did not reflect the findings in the brain. Nevertheless, the changes in the brain support the hypothesis that oxidative stress plays a role in neuronal damage after hypoxic stress, but the brain of IUGR did not reveal a special response to moderate hypoxia.