Ribosome-binding and anti-microbial studies of the mycinamicins, 16-membered macrolide antibiotics from Micromonospora griseorubida
Abstract Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the nascent protein exit tunnel in the ribosome. The macrolide class of natural products consist of a macrolactone ring linked to one or more sugar mo...
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Published in | Nucleic acids research Vol. 49; no. 16; pp. 9560 - 9573 |
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Main Authors | , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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Oxford University Press
20.09.2021
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Abstract | Abstract
Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the nascent protein exit tunnel in the ribosome. The macrolide class of natural products consist of a macrolactone ring linked to one or more sugar molecules. Most of the macrolides used currently are semi-synthetic erythromycin derivatives, composed of a 14- or 15-membered macrolactone ring. Rapidly emerging resistance in bacterial pathogens is among the most urgent global health challenges, which render many antibiotics ineffective, including next-generation macrolides. To address this threat and advance a longer-term plan for developing new antibiotics, we demonstrate how 16-membered macrolides overcome erythromycin resistance in clinically isolated Staphylococcus aureus strains. By determining the structures of complexes of the large ribosomal subunit of Deinococcus radiodurans (D50S) with these 16-membered selected macrolides, and performing anti-microbial studies, we identified resistance mechanisms they may overcome. This new information provides important insights toward the rational design of therapeutics that are effective against drug resistant human pathogens. |
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AbstractList | Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the nascent protein exit tunnel in the ribosome. The macrolide class of natural products consist of a macrolactone ring linked to one or more sugar molecules. Most of the macrolides used currently are semi-synthetic erythromycin derivatives, composed of a 14- or 15-membered macrolactone ring. Rapidly emerging resistance in bacterial pathogens is among the most urgent global health challenges, which render many antibiotics ineffective, including next-generation macrolides. To address this threat and advance a longer-term plan for developing new antibiotics, we demonstrate how 16-membered macrolides overcome erythromycin resistance in clinically isolated Staphylococcus aureus strains. By determining the structures of complexes of the large ribosomal subunit of Deinococcus radiodurans (D50S) with these 16-membered selected macrolides, and performing anti-microbial studies, we identified resistance mechanisms they may overcome. This new information provides important insights toward the rational design of therapeutics that are effective against drug resistant human pathogens. Abstract Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the nascent protein exit tunnel in the ribosome. The macrolide class of natural products consist of a macrolactone ring linked to one or more sugar molecules. Most of the macrolides used currently are semi-synthetic erythromycin derivatives, composed of a 14- or 15-membered macrolactone ring. Rapidly emerging resistance in bacterial pathogens is among the most urgent global health challenges, which render many antibiotics ineffective, including next-generation macrolides. To address this threat and advance a longer-term plan for developing new antibiotics, we demonstrate how 16-membered macrolides overcome erythromycin resistance in clinically isolated Staphylococcus aureus strains. By determining the structures of complexes of the large ribosomal subunit of Deinococcus radiodurans (D50S) with these 16-membered selected macrolides, and performing anti-microbial studies, we identified resistance mechanisms they may overcome. This new information provides important insights toward the rational design of therapeutics that are effective against drug resistant human pathogens. Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the nascent protein exit tunnel in the ribosome. The macrolide class of natural products consist of a macrolactone ring linked to one or more sugar molecules. Most of the macrolides used currently are semi-synthetic erythromycin derivatives, composed of a 14- or 15-membered macrolactone ring. Rapidly emerging resistance in bacterial pathogens is among the most urgent global health challenges, which render many antibiotics ineffective, including next-generation macrolides. To address this threat and advance a longer-term plan for developing new antibiotics, we demonstrate how 16-membered macrolides overcome erythromycin resistance in clinically isolated Staphylococcus aureus strains. By determining the structures of complexes of the large ribosomal subunit of Deinococcus radiodurans (D50S) with these 16-membered selected macrolides, and performing anti-microbial studies, we identified resistance mechanisms they may overcome. This new information provides important insights toward the rational design of therapeutics that are effective against drug resistant human pathogens. |
Author | Schmidt, Jennifer J Ezernitchi, Analia V Bashan, Anat Baum, Moti Lowell, Andrew N Eyal, Zohar Halfon, Yehuda Matzov, Donna Rozenberg, Haim Breiner-Goldstein, Elinor Rokney, Assaf Valinsky, Lea Sherman, David H Yonath, Ada Zimmerman, Ella Cimicata, Giuseppe Anzai, Yojiro |
Author_xml | – sequence: 1 givenname: Elinor surname: Breiner-Goldstein fullname: Breiner-Goldstein, Elinor – sequence: 2 givenname: Zohar orcidid: 0000-0002-8966-0624 surname: Eyal fullname: Eyal, Zohar – sequence: 3 givenname: Donna orcidid: 0000-0003-2600-9727 surname: Matzov fullname: Matzov, Donna – sequence: 4 givenname: Yehuda surname: Halfon fullname: Halfon, Yehuda – sequence: 5 givenname: Giuseppe surname: Cimicata fullname: Cimicata, Giuseppe – sequence: 6 givenname: Moti surname: Baum fullname: Baum, Moti – sequence: 7 givenname: Assaf surname: Rokney fullname: Rokney, Assaf – sequence: 8 givenname: Analia V surname: Ezernitchi fullname: Ezernitchi, Analia V – sequence: 9 givenname: Andrew N surname: Lowell fullname: Lowell, Andrew N – sequence: 10 givenname: Jennifer J surname: Schmidt fullname: Schmidt, Jennifer J – sequence: 11 givenname: Haim surname: Rozenberg fullname: Rozenberg, Haim – sequence: 12 givenname: Ella surname: Zimmerman fullname: Zimmerman, Ella – sequence: 13 givenname: Anat orcidid: 0000-0002-7705-0466 surname: Bashan fullname: Bashan, Anat – sequence: 14 givenname: Lea surname: Valinsky fullname: Valinsky, Lea – sequence: 15 givenname: Yojiro surname: Anzai fullname: Anzai, Yojiro – sequence: 16 givenname: David H surname: Sherman fullname: Sherman, David H – sequence: 17 givenname: Ada surname: Yonath fullname: Yonath, Ada email: ada.yonath@weizmann.ac.il |
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Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the... Macrolides have been effective clinical antibiotics for over 70 years. They inhibit protein biosynthesis in bacterial pathogens by narrowing the nascent... |
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Title | Ribosome-binding and anti-microbial studies of the mycinamicins, 16-membered macrolide antibiotics from Micromonospora griseorubida |
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