Antimicrobial activity of Annona muricata leaf oleoresin

• Published in: Natural product research Vol. 36; no. 18; pp. 4781 - 4787
• Main Authors: Caroline Zanella Cagnini, Angélica Barbosa Dias, Mariana Rodrigues Vilas Boas, Fabiana Pacheco Reis Batista, Maria Graciela Iecher Faria, Glamočlija, Jasmina, Soković, Marina, Tešević, Vele, Ederlan de Souza Ferreira, Nelson Barros Colauto, Giani, Andrea Linde, Zilda Cristiani Gazim
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Ltd 01.09.2022
• Subjects: Annona muricata; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Bacteria; Bactericidal activity; Bioactive compounds; Carbon dioxide; Chemical activity; Chemical composition; Enterobacter cloacae; Food industry; Fungicidal activity; Fungicides; Gas chromatography; Leaves; Mass spectrometry; Mass spectroscopy; Minimum inhibitory concentration; Oleoresins; Palmitic acid; Pharmaceutical industry; Phytol; Phytosterols; Plant extracts; Pseudomonas aeruginosa; Supercritical gas extraction; Tocopherol; Vitamin E
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2021.2011270

Bioactive compounds extracted from plants such as antimicrobials have attracted the attention of consumers and the food industry. This study aimed to determine the antimicrobial activity and chemical composition of Annona muricata leaf oleoresin obtained by supercritical CO2 extraction. The oleoresin was obtained by supercritical CO2 extraction and the chemical identification by gas chromatography coupled to mass spectrometry. Antimicrobial activity was evaluated by broth microdilution method against 14 foodborne fungi and bacteria. The oleoresin major chemical class was phytosterols (22.7%) and the major compounds were γ-sitosterol (15.7%), α-tocopherol (13.7%), phytol (13.1%), and hexadecanoic acid (11.5%). Minimum inhibitory concentration against bacteria ranged from 0.0025 to 0.010 mg mL−1. The oleoresin had high bactericidal activity against all bacteria, mainly Enterobacter cloacae and Pseudomonas aeruginosa with 0.005 mg mL−1 minimum bactericidal concentration. However, it had low fungicidal activity. The leaf oleoresin of A. muricata has promising applications in food, cosmetic, and pharmaceutical industries.