Mapping of peptidic epitopes of glycophorins A (GPA) and C (GPC) with peptides synthesized on plastic pins (Pepscan analysis)

• Published in: Transfusion clinique et biologique (Paris) Vol. 4; no. 1; pp. 73 - 75
• Main Authors: Waśniowska, K., Jaśkiewicz, E., Czerwiński, M., Syper, D., Lisowska, E.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Paris Elsevier SAS 1997; Elsevier
• Subjects: Antibodies, Monoclonal; Biological and medical sciences; Epitope Mapping; Epitopes; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Glycophorin - immunology; glycophorin A; glycophorin C; Glycosylation; Humans; Immunohematology; Peptide Mapping; Peptides - chemical synthesis; Plastics; Red blood cell immunology; synthetic peptides; glycophorin C; synthetic peptides; glycophorin A; Epitopes

The peptidic epitopes of 12 anti-GPA and 4 anti-GPC antibodies were identified with the use of peptides synthesized on the pins. Most of the antibodies were specific for epitopes located in extracellular portion of glycophorins, and only 2 anti-GPA and 1 anti-GPC recognized epitopes in their C-termi...