pegIT - a web-based design tool for prime editing

Abstract Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNAs (pegRNAs), serving both as guide molecu...

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Published inNucleic acids research Vol. 49; no. W1; pp. W505 - W509
Main Authors Anderson, Mads Valdemar, Haldrup, Jakob, Thomsen, Emil Aagaard, Wolff, Jonas Holst, Mikkelsen, Jacob Giehm
Format Journal Article
LanguageEnglish
Published Oxford University Press 02.07.2021
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Summary:Abstract Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNAs (pegRNAs), serving both as guide molecules and as templates carrying the desired edits. Due to such diverse functions, manual pegRNA design is a subject to error and not suited for large-scale setups. Here, we present pegIT, a user-friendly web tool for rapid pegRNA design for numerous user-defined edits, including large-scale setups. pegIT is freely available at https://pegit.giehmlab.dk. Graphical Abstract Graphical abstract pegIT is a freely available, user-friendly web tool that allows for easy and efficient pegRNA design for numerous, including large-scale, user-defined edits.
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ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkab427