Genetic structure of wild rice Oryza glumaepatula populations in three Brazilian biomes using microsatellite markers

• Published in: Genetica Vol. 125; no. 2-3; pp. 115 - 123
• Main Authors: Vianello Brondani, Rosana Pereira, Zucchi, Maria Imaculada, Brondani, Claudio, Nakano Rangel, Paulo Hideo, De Oliveira Borba, Tereza Cristina, Rangel, Priscila Nascimento, Magalhães, Mara Rubia, Vencovsky, Roland
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.11.2005
• Subjects: Alleles; Animal populations; Brazil; Conservation; DNA, Plant - genetics; Genetic diversity; Genetic markers; Genetic structure; Genetic Variation; Genetics, Population; Genome, Plant; Heterozygosity; Heterozygote; Homozygote; Homozygotes; Inbreeding; Microsatellite Repeats; Microsatellites; Oryza - genetics; Oryza glumaepatula; Oryza sativa; Preservation; Reproduction; Reproduction - genetics; Brazil
• ISSN: 0016-6707; 1573-6857
• DOI: 10.1007/s10709-005-4916-4

The existence of Oryza glumaepatula is threatened by devastation and, thus, the implementation of conservation strategies is extremely relevant. This study aimed to characterize the genetic variability and estimate population parameters of 30 O. glumaepatula populations from three Brazilian biomes using 10 microsatellite markers. The levels of allelic variability for the SSR loci presented a mean of 10.3 alleles per locus and a value of 0.10 for the average allelic frequency value. The expected total heterozygosity (H(e)) ranged from 0.63 to 0.86. For the 30 populations tested, the mean observed (H(o)) and expected heterozygosities (H(e)) were 0.03 and 0.11 within population, respectively, indicating an excess of homozygotes resulting from the preferentially self-pollinating reproduction habit. The estimated fixation index ( (IS) ) was 0.79 that differed significantly from zero, indicating high inbreeding within each O. glumaepatula population. The total inbreeding of the species ((IT) ) was 0.98 and the genetic diversity indexes among populations, (ST) and (ST), were 0.85 and 0.90, respectively, indicating high genetic variability among them. Thus, especially for populations located in regions threatened with devastation, it is urgent that in situ preservation conditions should be created or that collections be made for ex situ preservation to prevent loss of the species genetic variability.