An exponential dilution gradient system for nanoscale liquid chromatography in combination with MALDI or nano-ESI mass spectrometry for proteolytic digests

• Published in: Journal of the American Society for Mass Spectrometry Vol. 12; no. 11; pp. 1205 - 1213
• Main Authors: Doneanu, Catalin E, Griffin, Donald A, Barofsky, Elisabeth L, Barofsky, Douglas F
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2001; Springer Nature B.V
• Subjects: Chromatography; Chromatography, Liquid; Deoxyribonucleic acid; Desorption; Dilution; DNA; DNA - chemistry; E coli; Electrospraying; High performance liquid chromatography; Hydrolysis; Ionization; Ions; Lasers; Mass Spectrometry; Peptides; Peptides - chemistry; Proteins - chemistry; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet; Spectroscopy; Trypsin; Uracil

A simple and inexpensive nano high performance liquid chromatography system (nano-LC) employing the exponential dilution method for gradient separations was built. The system was used to analyze a tryptic digest of Escherichia coli uracil DNA glycosylase (Ung; M r = 25,563), a DNA-binding protein th...