An exponential dilution gradient system for nanoscale liquid chromatography in combination with MALDI or nano-ESI mass spectrometry for proteolytic digests

A simple and inexpensive nano high performance liquid chromatography system (nano-LC) employing the exponential dilution method for gradient separations was built. The system was used to analyze a tryptic digest of Escherichia coli uracil DNA glycosylase (Ung; M r = 25,563), a DNA-binding protein th...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Society for Mass Spectrometry Vol. 12; no. 11; pp. 1205 - 1213
Main Authors Doneanu, Catalin E, Griffin, Donald A, Barofsky, Elisabeth L, Barofsky, Douglas F
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.2001
Springer Nature B.V
Subjects
Online AccessGet full text

Cover

Loading…