Photofrin® increases murine spleen cell transferrin receptor expression and responsiveness to recombinant myeloid and erythroid growth factors

• Published in: Immunopharmacology Vol. 38; no. 3; pp. 267 - 278
• Main Authors: Hunt, David W.C, Jiang, Huijun, Levy, Julia G
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.1998; Elsevier
• Subjects: Animals; Antineoplastic Agents - pharmacology; Biological and medical sciences; Cell Cycle - drug effects; Cell Division - drug effects; Cell physiology; Colony-Stimulating Factors - pharmacology; Dihematoporphyrin Ether - pharmacology; Epidermal Growth Factor - pharmacology; Erythroid Precursor Cells - drug effects; Erythropoiesis; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Granulocyte-Macrophage Colony-Stimulating Factor - analysis; Hematopoiesis; Hematoporphyrins - pharmacology; Male; Medical sciences; Mice; Mice, Inbred DBA; Molecular and cellular biology; Organ Size - drug effects; Photofrin; Photoradiation therapy and photosensitizing agent; Photosensitizing Agents - pharmacology; Receptors, Interleukin-2 - drug effects; Receptors, Interleukin-2 - genetics; Receptors, Transferrin - biosynthesis; Receptors, Transferrin - drug effects; Recombinant Proteins - pharmacology; Responses to growth factors, tumor promotors, other factors; Spleen; Spleen - cytology; Spleen - drug effects; Spleen - metabolism; Transferrin receptor; Treatment with physical agents; Treatment. General aspects; Tumors; Erythropoiesis; PWM-SCCM, pokeweed mitogen spleen cell-conditioned medium; CFU-GM, colony forming unit, granulocyte-macrophage; HSA, heat stable antigen; TrfR, transferrin receptor; M-CSF, macrophage colony stimulating factor; HVD, hydroxyvinylethyl deuteroporphyrin; PBS, phosphate-buffered saline; CD, cluster of differentiation; Hematopoiesis; IMDM, Iscove's modified Dulbecco's medium; BFU-E, burst forming unit, erythroid; Spleen; BSA, bovine serum albumin; GM-CSF, granulocyte-macrophage colony stimulating factor; G-CSF, granulocyte colony stimulating factor; PI, propidium iodide; FITC, fluorescein isothiocyanate; MTT, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; Photofrin; Hp, hematoporphyrin; r, recombinant; Transferrin receptor; 2-ME, 2-mercaptoethanol; PE, phycoerythrin; CFU-E, colony forming unit, erythroid; i.p., intraperitoneal; IL, interleukin; IL-2R, interleukin-2 receptor; Epo, erythropoietin; FACS, fluorescence-activated cell sorter analysis; FCS, fetal calf serum; Transferrin; Intraperitoneal administration; Rodentia; Cytokine; Gene expression; Hematoporphyrin; Colony forming cell; Phototherapy; Porfimer sodium; Vertebrata; Mammalia; Treatment; Interleukin 2; Mouse; Porphyrin; Animal; Recombinant protein; Photosensitizer; Growth factor; Biological receptor

When given to normal male mice, the photochemotherapeutic agent Photofrin® (porfimer sodium), a complex mixture of monomeric and oligomeric non-metallic porphyrins, significantly increased relative spleen weight, spleen cell numbers, DNA replication, levels of granulocyte-macrophage and erythroid pr...