Sequencing and characterization of miRNAs and mRNAs from the longissimus dorsi of Xinjiang brown cattle and Kazakh cattle
•The results showed that 1669 genes were differentially expressed.•Additionally, 346 differentially expressed miRNAs were identified.•The expression levels of 19 genes and miRNAs were verified by qRT-PCR. Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh...
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| Published in | Gene Vol. 741; p. 144537 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier B.V
30.05.2020
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| Abstract | •The results showed that 1669 genes were differentially expressed.•Additionally, 346 differentially expressed miRNAs were identified.•The expression levels of 19 genes and miRNAs were verified by qRT-PCR.
Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh cattle, Xinjiang brown cattle have significantly improved carcass quality and meat quality. To elucidate the molecular mechanisms underlying the improvements in Xinjiang brown cattle and the differences in beef quality between the two breeds, we used RNA-Seq to study differentially expressed genes and miRNAs and regulatory pathways related to adipogenesis, myogenesis and fibrogenesis in the longissimus dorsi muscles of Xinjiang brown cattle and Kazakh cattle. The results showed that 1669 genes were differentially expressed in the longissimus dorsi muscle tissues of Xinjiang brown cattle and Kazakh cattle; 879 genes were upregulated and 790 genes were downregulated in Xinjiang brown cattle compared to Kazakh cattle. These genes were mainly involved in PPAR signaling, unsaturated fatty acid biosynthesis and vascular smooth muscle contraction. Additionally, 346 differentially expressed miRNAs were identified, of which 265 miRNAs were downregulated and 81 miRNAs were upregulated in Xinjiang brown cattle compared to Kazakh cattle. Association analysis of the differentially expressed genes and miRNAs revealed that 86 differentially expressed miRNAs related to adipogenesis were associated with 31 differentially expressed genes, 76 differentially expressed miRNAs associated with myogenesis were associated with 28 differentially expressed genes, and 54 differentially expressed miRNAs associated with fibrogenesis were associated with 19 differentially expressed genes. miRNA-target gene networks were also constructed. Finally, the expression levels of 19 genes and miRNAs were verified by qRT-PCR. Some differentially expressed genes, including FABP4, ACTA2 and ACTG2, were shown to play an important role in beef meat quality. This is the first study to perform transcriptomic analysis of muscle tissues from Xinjiang brown and Kazakh cattle. |
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| AbstractList | Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh cattle, Xinjiang brown cattle have significantly improved carcass quality and meat quality. To elucidate the molecular mechanisms underlying the improvements in Xinjiang brown cattle and the differences in beef quality between the two breeds, we used RNA-Seq to study differentially expressed genes and miRNAs and regulatory pathways related to adipogenesis, myogenesis and fibrogenesis in the longissimus dorsi muscles of Xinjiang brown cattle and Kazakh cattle. The results showed that 1669 genes were differentially expressed in the longissimus dorsi muscle tissues of Xinjiang brown cattle and Kazakh cattle; 879 genes were upregulated and 790 genes were downregulated in Xinjiang brown cattle compared to Kazakh cattle. These genes were mainly involved in PPAR signaling, unsaturated fatty acid biosynthesis and vascular smooth muscle contraction. Additionally, 346 differentially expressed miRNAs were identified, of which 265 miRNAs were downregulated and 81 miRNAs were upregulated in Xinjiang brown cattle compared to Kazakh cattle. Association analysis of the differentially expressed genes and miRNAs revealed that 86 differentially expressed miRNAs related to adipogenesis were associated with 31 differentially expressed genes, 76 differentially expressed miRNAs associated with myogenesis were associated with 28 differentially expressed genes, and 54 differentially expressed miRNAs associated with fibrogenesis were associated with 19 differentially expressed genes. miRNA-target gene networks were also constructed. Finally, the expression levels of 19 genes and miRNAs were verified by qRT-PCR. Some differentially expressed genes, including FABP4, ACTA2 and ACTG2, were shown to play an important role in beef meat quality. This is the first study to perform transcriptomic analysis of muscle tissues from Xinjiang brown and Kazakh cattle. Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh cattle, Xinjiang brown cattle have significantly improved carcass quality and meat quality. To elucidate the molecular mechanisms underlying the improvements in Xinjiang brown cattle and the differences in beef quality between the two breeds, we used RNA-Seq to study differentially expressed genes and miRNAs and regulatory pathways related to adipogenesis, myogenesis and fibrogenesis in the longissimus dorsi muscles of Xinjiang brown cattle and Kazakh cattle. The results showed that 1669 genes were differentially expressed in the longissimus dorsi muscle tissues of Xinjiang brown cattle and Kazakh cattle; 879 genes were upregulated and 790 genes were downregulated in Xinjiang brown cattle compared to Kazakh cattle. These genes were mainly involved in PPAR signaling, unsaturated fatty acid biosynthesis and vascular smooth muscle contraction. Additionally, 346 differentially expressed miRNAs were identified, of which 265 miRNAs were downregulated and 81 miRNAs were upregulated in Xinjiang brown cattle compared to Kazakh cattle. Association analysis of the differentially expressed genes and miRNAs revealed that 86 differentially expressed miRNAs related to adipogenesis were associated with 31 differentially expressed genes, 76 differentially expressed miRNAs associated with myogenesis were associated with 28 differentially expressed genes, and 54 differentially expressed miRNAs associated with fibrogenesis were associated with 19 differentially expressed genes. miRNA-target gene networks were also constructed. Finally, the expression levels of 19 genes and miRNAs were verified by qRT-PCR. Some differentially expressed genes, including FABP4, ACTA2 and ACTG2, were shown to play an important role in beef meat quality. This is the first study to perform transcriptomic analysis of muscle tissues from Xinjiang brown and Kazakh cattle.Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh cattle, Xinjiang brown cattle have significantly improved carcass quality and meat quality. To elucidate the molecular mechanisms underlying the improvements in Xinjiang brown cattle and the differences in beef quality between the two breeds, we used RNA-Seq to study differentially expressed genes and miRNAs and regulatory pathways related to adipogenesis, myogenesis and fibrogenesis in the longissimus dorsi muscles of Xinjiang brown cattle and Kazakh cattle. The results showed that 1669 genes were differentially expressed in the longissimus dorsi muscle tissues of Xinjiang brown cattle and Kazakh cattle; 879 genes were upregulated and 790 genes were downregulated in Xinjiang brown cattle compared to Kazakh cattle. These genes were mainly involved in PPAR signaling, unsaturated fatty acid biosynthesis and vascular smooth muscle contraction. Additionally, 346 differentially expressed miRNAs were identified, of which 265 miRNAs were downregulated and 81 miRNAs were upregulated in Xinjiang brown cattle compared to Kazakh cattle. Association analysis of the differentially expressed genes and miRNAs revealed that 86 differentially expressed miRNAs related to adipogenesis were associated with 31 differentially expressed genes, 76 differentially expressed miRNAs associated with myogenesis were associated with 28 differentially expressed genes, and 54 differentially expressed miRNAs associated with fibrogenesis were associated with 19 differentially expressed genes. miRNA-target gene networks were also constructed. Finally, the expression levels of 19 genes and miRNAs were verified by qRT-PCR. Some differentially expressed genes, including FABP4, ACTA2 and ACTG2, were shown to play an important role in beef meat quality. This is the first study to perform transcriptomic analysis of muscle tissues from Xinjiang brown and Kazakh cattle. •The results showed that 1669 genes were differentially expressed.•Additionally, 346 differentially expressed miRNAs were identified.•The expression levels of 19 genes and miRNAs were verified by qRT-PCR. Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh cattle, Xinjiang brown cattle have significantly improved carcass quality and meat quality. To elucidate the molecular mechanisms underlying the improvements in Xinjiang brown cattle and the differences in beef quality between the two breeds, we used RNA-Seq to study differentially expressed genes and miRNAs and regulatory pathways related to adipogenesis, myogenesis and fibrogenesis in the longissimus dorsi muscles of Xinjiang brown cattle and Kazakh cattle. The results showed that 1669 genes were differentially expressed in the longissimus dorsi muscle tissues of Xinjiang brown cattle and Kazakh cattle; 879 genes were upregulated and 790 genes were downregulated in Xinjiang brown cattle compared to Kazakh cattle. These genes were mainly involved in PPAR signaling, unsaturated fatty acid biosynthesis and vascular smooth muscle contraction. Additionally, 346 differentially expressed miRNAs were identified, of which 265 miRNAs were downregulated and 81 miRNAs were upregulated in Xinjiang brown cattle compared to Kazakh cattle. Association analysis of the differentially expressed genes and miRNAs revealed that 86 differentially expressed miRNAs related to adipogenesis were associated with 31 differentially expressed genes, 76 differentially expressed miRNAs associated with myogenesis were associated with 28 differentially expressed genes, and 54 differentially expressed miRNAs associated with fibrogenesis were associated with 19 differentially expressed genes. miRNA-target gene networks were also constructed. Finally, the expression levels of 19 genes and miRNAs were verified by qRT-PCR. Some differentially expressed genes, including FABP4, ACTA2 and ACTG2, were shown to play an important role in beef meat quality. This is the first study to perform transcriptomic analysis of muscle tissues from Xinjiang brown and Kazakh cattle. |
| ArticleNumber | 144537 |
| Author | Li, Na Zhang, Yang Li, Hong-Bo Yan, Xiang-Ming Yu, Qun-Li |
| Author_xml | – sequence: 1 givenname: Na surname: Li fullname: Li, Na organization: Department of Research Livestock, Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China – sequence: 2 givenname: Qun-Li surname: Yu fullname: Yu, Qun-Li organization: Department of Food Science and Engineering, Gansu Agricultural University, Lanzhou, Gansu 730070, China – sequence: 3 givenname: Xiang-Ming surname: Yan fullname: Yan, Xiang-Ming organization: Department of Research Livestock, Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China – sequence: 4 givenname: Hong-Bo surname: Li fullname: Li, Hong-Bo organization: Department of Research Livestock, Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China – sequence: 5 givenname: Yang surname: Zhang fullname: Zhang, Yang email: zhyang1962@126.com organization: Department of Research Livestock, Xinjiang Academy of Animal Science, Urumqi, Xinjiang 830000, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32156528$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/j.cmet.2008.12.014 10.1073/pnas.101042998 10.1016/j.febslet.2011.09.015 10.1016/j.gene.2015.02.070 10.1242/jcs.010728 10.2527/jas.2012-5892 10.2527/jas.2011-4937 10.2527/2001.79102605x 10.2527/jas.2012-5670 10.5713/ajas.14.0394 10.1128/JVI.01156-09 10.1016/j.jmb.2007.01.045 10.1371/journal.pone.0064356 10.1038/nmeth.1923 10.1038/ng0997-71 10.1093/bioinformatics/btp612 10.1038/ncb2286 10.1073/pnas.1102281108 10.1186/1471-2105-12-323 10.1016/j.livsci.2010.06.070 10.1038/nbt1394 10.4238/2013.January.4.21 10.3724/SP.J.1005.2009.00489 |
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| Keywords | miRNAs PUFA Differentially expressed gene GO MHC IGF mRNA Xinjiang brown cattle FPKM FGFR Longissimus dorsi CAM FGFs IGFBP Kazakh DEGs FABPs RBP4 HCM KEGG miRNA ASRA |
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| References | Yi, Xie, Li, Lv, He, Wu (b0140) 2011; 585 Suggs, Kronert, Nikkhoy, Dambacher, Megighian, Bernstein (b0105) 2007; 367 Huang, Deng (b0100) 2009; 31 Linmin Sun, Xiang (b0005) 2015; 402 Li, Dewey (b0045) 2011; 12 Wei, Wang, Cheng, Du, Jiang, Hausman, McFarland, Dodson (b0085) 2013; 12 Norman (b0135) 2010; 84 Wang, Wang, Wang, Zhang (b0050) 2010; 21 Cousin, Morroni, Raimbault, Ricquier, Pénicaud, Casteilla (b0130) 1992; 931–42 Driver, Huang, Ahmad, Hackbart, Wiltbank, Khatib (b0070) 2012; 13 Langmead (b0040) 2012; 9 Shackelford, Meade, Reagan, Byrnes, Koohmaraie (b0020) 2001; 79 Chong Tang YX, Wei Yan. AASRA: An Anchor Alignment-Based Small RNA Annotation Pipeline. bioRxiv. Du, Das, Yang, Duarte, Dodson, Zhu (b0030) 2013; 91 Sun, Mori, Alexander, Yuan, Hattangadi, Liu, Kahn, Lodish (b0120) 2011; 13 Xiangyang, Xiaoyu (b0015) 2015; 562 Feldmann, Cannon, Nedergaard (b0125) 2009; 9 Xu, Pan, Chu, Luo, Lin, Xiao, Shan, Wang, Yang (b0145) 2007; 120 Chen, Li, Du, Wu, Cao (b0010) 2015; 28 Duarte, Das, Wei, Serão, Fu, Harris, Dodson, Du (b0090) 2013; 91 Dávalos, Smibert, Ramírez, Warrier, Andreo, Cirera-Salinas, Rayner, Suresh, Pastor-Pareja, Esplugues, Fisher, Penalva, Moore, Suárez, Lai, Fernández-Hernando (b0115) 2011; 108 Grobet, Poncelet, Pirottin, Brouwers, Riquet, Schoeberlein, Dunner, Ménissier, Massabanda, Fries, Hanset, Georges (b0025) 1997; 17 He (b0065) 2013; 8 Friedländer, Adamidi, Maaskola, Einspanier, Knespel, Rajewsky (b0060) 2008; 26 Knudsen (b0075) 1990; 98 Zhang, Wang (b0110) 2011; 135 Du (b0035) 2012; 90 Luiken, van Nieuwenhoven, van der Vusse, Bonen, Glatz (b0080) 1990; S169–75 Tansey, Gruia-Gray, Roush, Zee, Gavrilova, Reitman, Deng, Li, Kimmel, Londos (b0095) 2001; 98 10.1016/j.gene.2020.144537_b0055 Du (10.1016/j.gene.2020.144537_b0030) 2013; 91 He (10.1016/j.gene.2020.144537_b0065) 2013; 8 Friedländer (10.1016/j.gene.2020.144537_b0060) 2008; 26 Tansey (10.1016/j.gene.2020.144537_b0095) 2001; 98 Yi (10.1016/j.gene.2020.144537_b0140) 2011; 585 Zhang (10.1016/j.gene.2020.144537_b0110) 2011; 135 Chen (10.1016/j.gene.2020.144537_b0010) 2015; 28 Shackelford (10.1016/j.gene.2020.144537_b0020) 2001; 79 Norman (10.1016/j.gene.2020.144537_b0135) 2010; 84 Feldmann (10.1016/j.gene.2020.144537_b0125) 2009; 9 Linmin Sun (10.1016/j.gene.2020.144537_b0005) 2015; 402 Duarte (10.1016/j.gene.2020.144537_b0090) 2013; 91 Wang (10.1016/j.gene.2020.144537_b0050) 2010; 21 Wei (10.1016/j.gene.2020.144537_b0085) 2013; 12 Cousin (10.1016/j.gene.2020.144537_b0130) 1992; 931–42 Knudsen (10.1016/j.gene.2020.144537_b0075) 1990; 98 Driver (10.1016/j.gene.2020.144537_b0070) 2012; 13 Xu (10.1016/j.gene.2020.144537_b0145) 2007; 120 Langmead (10.1016/j.gene.2020.144537_b0040) 2012; 9 Sun (10.1016/j.gene.2020.144537_b0120) 2011; 13 Grobet (10.1016/j.gene.2020.144537_b0025) 1997; 17 Du (10.1016/j.gene.2020.144537_b0035) 2012; 90 Xiangyang (10.1016/j.gene.2020.144537_b0015) 2015; 562 Suggs (10.1016/j.gene.2020.144537_b0105) 2007; 367 Dávalos (10.1016/j.gene.2020.144537_b0115) 2011; 108 Li (10.1016/j.gene.2020.144537_b0045) 2011; 12 Huang (10.1016/j.gene.2020.144537_b0100) 2009; 31 Luiken (10.1016/j.gene.2020.144537_b0080) 1990; S169–75 |
| References_xml | – volume: 26 start-page: 407 year: 2008 end-page: 415 ident: b0060 article-title: Discovering microRNAs from deep sequencing data using miRDeep publication-title: Nat. Biotechnol. – volume: 12 start-page: 494 year: 2013 end-page: 505 ident: b0085 article-title: Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes publication-title: Genet. Mol. Res. – volume: 931–42 year: 1992 ident: b0130 article-title: Occurrence of brown adipocytes in rat white adipose tissue: molecular and morphological characterization publication-title: J. Cell Sci. – reference: Chong Tang YX, Wei Yan. AASRA: An Anchor Alignment-Based Small RNA Annotation Pipeline. bioRxiv. – volume: 98 start-page: 217 year: 1990 end-page: 223 ident: b0075 article-title: Acyl-CoA-binding protein (ACBP) and its relation to fatty acid-binding protein (FABP): an overview publication-title: Mol. Cell. Biochem. – volume: 31 start-page: 489 year: 2009 end-page: 494 ident: b0100 article-title: Cloning and expression analysis of porcine ACTA2 gene and its association with production traits publication-title: Yi Chuan. – volume: 91 start-page: 2938 year: 2013 end-page: 2946 ident: b0090 article-title: Enhancement of adipogenesis and fibrogenesis in skeletal muscle of Wagyu compared with Angus cattle publication-title: J. Anim. Sci. – volume: 21 start-page: 136 year: 2010 end-page: 138 ident: b0050 article-title: DEGseq: an R package for identifying differentially expressed genes from RNA-seq data publication-title: Bioinformatics – volume: 120 start-page: 3045 year: 2007 end-page: 3052 ident: b0145 article-title: The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes publication-title: J. Cell Sci. – volume: 367 start-page: 1312 year: 2007 end-page: 1329 ident: b0105 article-title: Alternative S2 hinge regions of the myosin rod differentially affect muscle function, myofibril dimensions and myosin tail length publication-title: J. Mol. Biol. – volume: 108 start-page: 9232 year: 2011 end-page: 9237 ident: b0115 article-title: miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling publication-title: Proc. Natl. Acad. Sci. U.S.A. – volume: 585 start-page: 3303 year: 2011 end-page: 3309 ident: b0140 article-title: MiR-143 enhances adipogenic differentiation of 3T3-L1 cells through targeting the coding region of mouse pleiotrophin publication-title: FEBS Lett. – volume: 90 start-page: 922 year: 2012 end-page: 923 ident: b0035 article-title: Meat Science and Muscle Biology Symposium: extracellular matrix in skeletal muscle development and meat quality publication-title: J. Anim. Sci. – volume: 28 start-page: 158 year: 2015 end-page: 165 ident: b0010 article-title: Sequencing and characterization of divergent marbling levels in the beef cattle (Longissimus dorsi Muscle) transcriptome publication-title: Asian-Australas J. Anim. Sci. – volume: 17 start-page: 71 year: 1997 end-page: 74 ident: b0025 article-title: A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle publication-title: Nat. Genet. – volume: 12 year: 2011 ident: b0045 article-title: RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome publication-title: BMC Bioinf. – volume: 562 start-page: 188 year: 2015 end-page: 196 ident: b0015 article-title: Genome-wide analysis reveals the differential regulations of mRNAs and miRNAs in Dorset and Small Tail Han sheep muscles publication-title: Gene – volume: 98 start-page: 6494 year: 2001 end-page: 6499 ident: b0095 article-title: Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity publication-title: Proc. Natl. Acad. Sci. U.S.A. – volume: 402 start-page: 32 year: 2015 end-page: 42 ident: b0005 article-title: Comparative transcriptome profiling of longissmus muscle tissues from Qianhua Mutton Merino and Small Tai Han sheep publication-title: Sci. Rep. – volume: 8 year: 2013 ident: b0065 article-title: Characterization of transcriptional complexity during longissimus muscle development in bovines using high-throughput sequencing publication-title: PLoS ONE – volume: 91 start-page: 1419 year: 2013 end-page: 1427 ident: b0030 article-title: Meat Science and Muscle Biology Symposium: manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle publication-title: J. Anim. Sci. – volume: 13 year: 2012 ident: b0070 article-title: RNA-Seq analysis uncovers transcriptomic variations between morphologically similar in vivo- and in vitro-derived bovine blastocysts publication-title: BMC Genomics – volume: S169–75 year: 1990 ident: b0080 article-title: Cellular fatty acid transport in heart and skeletal muscle as facilitated by proteins publication-title: Lipids – volume: 135 start-page: 76 year: 2011 end-page: 83 ident: b0110 article-title: Differentially expressed genes in skeletal muscle tissues from castrated Qinchuan cattle males compared with those from intact males publication-title: Livestock Sci. – volume: 13 start-page: 958 year: 2011 end-page: 965 ident: b0120 article-title: Mir193b-365 is essential for brown fat differentiation publication-title: Nat. Cell Biol. – volume: 9 start-page: 203 year: 2009 end-page: 209 ident: b0125 article-title: UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality publication-title: Cell Metab. – volume: 79 start-page: 2605 year: 2001 end-page: 2614 ident: b0020 article-title: Consumer impressions of Tender Select beef publication-title: J. Anim. Sci. – volume: 84 start-page: 666 year: 2010 end-page: 670 ident: b0135 article-title: Modulation of hepatitis C virus RNA abundance and the isoprenoid biosynthesis pathway by microRNA miR-122 involves distinct mechanisms publication-title: J. Virol. – volume: 9 start-page: 357 year: 2012 end-page: 359 ident: b0040 article-title: Fast gapped-read alignment with Bowtie 2 publication-title: Nat. Methods – volume: 9 start-page: 203 issue: 2 year: 2009 ident: 10.1016/j.gene.2020.144537_b0125 article-title: UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality publication-title: Cell Metab. doi: 10.1016/j.cmet.2008.12.014 – ident: 10.1016/j.gene.2020.144537_b0055 – volume: 98 start-page: 6494 issue: 11 year: 2001 ident: 10.1016/j.gene.2020.144537_b0095 article-title: Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.101042998 – volume: 13 issue: 118 year: 2012 ident: 10.1016/j.gene.2020.144537_b0070 article-title: RNA-Seq analysis uncovers transcriptomic variations between morphologically similar in vivo- and in vitro-derived bovine blastocysts publication-title: BMC Genomics – volume: 585 start-page: 3303 issue: 20 year: 2011 ident: 10.1016/j.gene.2020.144537_b0140 article-title: MiR-143 enhances adipogenic differentiation of 3T3-L1 cells through targeting the coding region of mouse pleiotrophin publication-title: FEBS Lett. doi: 10.1016/j.febslet.2011.09.015 – volume: 562 start-page: 188 issue: 2 year: 2015 ident: 10.1016/j.gene.2020.144537_b0015 article-title: Genome-wide analysis reveals the differential regulations of mRNAs and miRNAs in Dorset and Small Tail Han sheep muscles publication-title: Gene doi: 10.1016/j.gene.2015.02.070 – volume: 120 start-page: 3045 issue: Pt 17 year: 2007 ident: 10.1016/j.gene.2020.144537_b0145 article-title: The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes publication-title: J. Cell Sci. doi: 10.1242/jcs.010728 – volume: 91 start-page: 2938 issue: 6 year: 2013 ident: 10.1016/j.gene.2020.144537_b0090 article-title: Enhancement of adipogenesis and fibrogenesis in skeletal muscle of Wagyu compared with Angus cattle publication-title: J. Anim. Sci. doi: 10.2527/jas.2012-5892 – volume: 90 start-page: 922 year: 2012 ident: 10.1016/j.gene.2020.144537_b0035 article-title: Meat Science and Muscle Biology Symposium: extracellular matrix in skeletal muscle development and meat quality publication-title: J. Anim. Sci. doi: 10.2527/jas.2011-4937 – volume: S169–75 year: 1990 ident: 10.1016/j.gene.2020.144537_b0080 article-title: Cellular fatty acid transport in heart and skeletal muscle as facilitated by proteins publication-title: Lipids – volume: 79 start-page: 2605 issue: 10 year: 2001 ident: 10.1016/j.gene.2020.144537_b0020 article-title: Consumer impressions of Tender Select beef publication-title: J. Anim. Sci. doi: 10.2527/2001.79102605x – volume: 91 start-page: 1419 issue: 3 year: 2013 ident: 10.1016/j.gene.2020.144537_b0030 article-title: Meat Science and Muscle Biology Symposium: manipulating mesenchymal progenitor cell differentiation to optimize performance and carcass value of beef cattle publication-title: J. Anim. Sci. doi: 10.2527/jas.2012-5670 – volume: 28 start-page: 158 issue: 2 year: 2015 ident: 10.1016/j.gene.2020.144537_b0010 article-title: Sequencing and characterization of divergent marbling levels in the beef cattle (Longissimus dorsi Muscle) transcriptome publication-title: Asian-Australas J. Anim. Sci. doi: 10.5713/ajas.14.0394 – volume: 84 start-page: 666 issue: 1 year: 2010 ident: 10.1016/j.gene.2020.144537_b0135 article-title: Modulation of hepatitis C virus RNA abundance and the isoprenoid biosynthesis pathway by microRNA miR-122 involves distinct mechanisms publication-title: J. Virol. doi: 10.1128/JVI.01156-09 – volume: 402 start-page: 32 year: 2015 ident: 10.1016/j.gene.2020.144537_b0005 article-title: Comparative transcriptome profiling of longissmus muscle tissues from Qianhua Mutton Merino and Small Tai Han sheep publication-title: Sci. Rep. – volume: 98 start-page: 217 issue: 1–2 year: 1990 ident: 10.1016/j.gene.2020.144537_b0075 article-title: Acyl-CoA-binding protein (ACBP) and its relation to fatty acid-binding protein (FABP): an overview publication-title: Mol. Cell. Biochem. – volume: 367 start-page: 1312 issue: 5 year: 2007 ident: 10.1016/j.gene.2020.144537_b0105 article-title: Alternative S2 hinge regions of the myosin rod differentially affect muscle function, myofibril dimensions and myosin tail length publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2007.01.045 – volume: 8 issue: 6 year: 2013 ident: 10.1016/j.gene.2020.144537_b0065 article-title: Characterization of transcriptional complexity during longissimus muscle development in bovines using high-throughput sequencing publication-title: PLoS ONE doi: 10.1371/journal.pone.0064356 – volume: 9 start-page: 357 issue: 4 year: 2012 ident: 10.1016/j.gene.2020.144537_b0040 article-title: Fast gapped-read alignment with Bowtie 2 publication-title: Nat. Methods doi: 10.1038/nmeth.1923 – volume: 17 start-page: 71 issue: 1 year: 1997 ident: 10.1016/j.gene.2020.144537_b0025 article-title: A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle publication-title: Nat. Genet. doi: 10.1038/ng0997-71 – volume: 21 start-page: 136 issue: 6 year: 2010 ident: 10.1016/j.gene.2020.144537_b0050 article-title: DEGseq: an R package for identifying differentially expressed genes from RNA-seq data publication-title: Bioinformatics doi: 10.1093/bioinformatics/btp612 – volume: 13 start-page: 958 issue: 8 year: 2011 ident: 10.1016/j.gene.2020.144537_b0120 article-title: Mir193b-365 is essential for brown fat differentiation publication-title: Nat. Cell Biol. doi: 10.1038/ncb2286 – volume: 108 start-page: 9232 issue: 22 year: 2011 ident: 10.1016/j.gene.2020.144537_b0115 article-title: miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.1102281108 – volume: 12 issue: 1 year: 2011 ident: 10.1016/j.gene.2020.144537_b0045 article-title: RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome publication-title: BMC Bioinf. doi: 10.1186/1471-2105-12-323 – volume: 135 start-page: 76 issue: 1 year: 2011 ident: 10.1016/j.gene.2020.144537_b0110 article-title: Differentially expressed genes in skeletal muscle tissues from castrated Qinchuan cattle males compared with those from intact males publication-title: Livestock Sci. doi: 10.1016/j.livsci.2010.06.070 – volume: 26 start-page: 407 issue: 4 year: 2008 ident: 10.1016/j.gene.2020.144537_b0060 article-title: Discovering microRNAs from deep sequencing data using miRDeep publication-title: Nat. Biotechnol. doi: 10.1038/nbt1394 – volume: 12 start-page: 494 issue: 1 year: 2013 ident: 10.1016/j.gene.2020.144537_b0085 article-title: Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes publication-title: Genet. Mol. Res. doi: 10.4238/2013.January.4.21 – volume: 931–42 year: 1992 ident: 10.1016/j.gene.2020.144537_b0130 article-title: Occurrence of brown adipocytes in rat white adipose tissue: molecular and morphological characterization publication-title: J. Cell Sci. – volume: 31 start-page: 489 issue: 5 year: 2009 ident: 10.1016/j.gene.2020.144537_b0100 article-title: Cloning and expression analysis of porcine ACTA2 gene and its association with production traits publication-title: Yi Chuan. doi: 10.3724/SP.J.1005.2009.00489 |
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| Snippet | •The results showed that 1669 genes were differentially expressed.•Additionally, 346 differentially expressed miRNAs were identified.•The expression levels of... Breed improvement is an important genetic process affecting meat quality. Compared with Kazakh cattle, Xinjiang brown cattle have significantly improved... |
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| SubjectTerms | adipogenesis Animals beef beef quality biosynthesis Breeding breeds carcass quality cattle Cattle - genetics Cattle - growth & development China Differentially expressed gene fatty acids Gene Expression Profiling gene expression regulation Gene Expression Regulation, Developmental - genetics gene regulatory networks Gene Regulatory Networks - genetics genes Kazakh Longissimus dorsi longissimus muscle Meat microRNA MicroRNAs - genetics miRNA mRNA muscle contraction muscle development Muscle Development - genetics Muscle, Skeletal - growth & development peroxisome proliferator-activated receptors RNA, Messenger - genetics sequence analysis Sequence Analysis, RNA smooth muscle Transcriptome - genetics transcriptomics Xinjiang brown cattle |
| Title | Sequencing and characterization of miRNAs and mRNAs from the longissimus dorsi of Xinjiang brown cattle and Kazakh cattle |
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