Sensitive determination of anatoxin-a, homoanatoxin-a and their degradation products by liquid chromatography with fluorimetric detection

• Published in: Journal of Chromatography A Vol. 798; no. 1; pp. 147 - 157
• Main Authors: James, Kevin J, Furey, Ambrose, Sherlock, Ian R, Stack, Mary A, Twohig, Marian, Caudwell, F.Barry, Skulberg, Olav M
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 06.03.1998; Elsevier
• Subjects: Anatoxins; Bacterial Toxins - analysis; Bacteriology; Biological and medical sciences; Bridged Bicyclo Compounds, Heterocyclic - analysis; Chromatography, High Pressure Liquid - methods; Cyanobacteria - chemistry; Fluorometry; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Homoanatoxins; Marine Toxins - analysis; Microbiology; Microcystins; Neurotoxins; Neurotoxins - analysis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Toxins; Tropanes; Europe; Ireland; Anatoxins; Neurotoxins; Toxins; Derivatization, LC; Water analysis; Homoanatoxins; Solid phase extraction; Chemical analysis; Nitrogen heterocycle; Metabolite; Toxoid; HPLC chromatography; Derivatization; Freshwater environment; Alkaloid; Sample preparation; Cyanobacteria; Bicyclic compound; Bacteria; Qualitative analysis; Oscillatoria; Quantitative analysis; Fluorescence detector; Ketone; Toxin; Reversed phase chromatography; Analysis method; Microbial origin; Fluorescent labelling; Epoxide; Neurotoxin; Lakes; Degradation product

Cyanobacterial neurotoxins have been implicated in animal deaths resulting from drinking contaminated water. Anatoxin-a (AN) and homoanatoxin-a (HMAN) have previously been analysed using high-performance liquid chromatography (HPLC) with UV detection, but this procedure is insufficiently sensitive a...