Laser Doppler holography of the anterior segment for blood flow imaging, eye tracking, and transparency assessment

• Published in: Biomedical optics express Vol. 12; no. 7; pp. 4478 - 4495
• Main Authors: Puyo, Léo, David, Clémentine, Saad, Rana, Saad, Sami, Gautier, Josselin, Sahel, José Alain, Borderie, Vincent, Paques, Michel, Atlan, Michael
• Format: Journal Article
• Language: English
• Published: Optical Society of America - OSA Publishing 01.07.2021; Optical Society of America
• Subjects: Life Sciences
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/BOE.425272

Laser Doppler holography (LDH) is a full-field blood flow imaging technique able to reveal human retinal and choroidal blood flow with high temporal resolution. We here report on using LDH in the anterior segment of the eye without making changes to the instrument. Blood flow in the bulbar conjunctiva and episclera as well as in corneal neovascularization can be effectively imaged. We additionally demonstrate simultaneous holographic imaging of the anterior and posterior segments by simply adapting the numerical propagation distance to the plane of interest. We used this feature to track the movements of the retina and pupil with high temporal resolution. Finally, we show that the light backscattered by the retina can be used for retro-illumination of the anterior segment. Hence digital holography can reveal opacities caused by absorption or diffusion in the cornea and eye lens.