Adult zebrafish model of streptococcal infection
Streptococcal pathogens cause a wide array of clinical syndromes in humans, including invasive systemic infections resulting in high mortality rates. Many of these pathogens are human specific, and therefore difficult to analyze in vivo using typical animal models, as these models rarely replicate w...
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Published in | Current protocols in microbiology (Online) Vol. Chapter 9; p. Unit 9D.1 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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United States
01.05.2009
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Abstract | Streptococcal pathogens cause a wide array of clinical syndromes in humans, including invasive systemic infections resulting in high mortality rates. Many of these pathogens are human specific, and therefore difficult to analyze in vivo using typical animal models, as these models rarely replicate what is observed in human infections. This unit describes the use of the zebrafish (Danio rerio) as an animal model for streptococcal infection to analyze multiple disease states. This model closely mimics the necrotizing fasciitis/myositis pathology observed in humans from a Streptococcus pyogenes infection. The use of a zoonotic pathogen, Streptococcus iniae, which replicates systemic infections caused by many streptococcal pathogens, including dissemination to the brain, is also described. Protocols describing both intraperitoneal and intramuscular infections, as well as methods for histological and quantitative measurements of infection, are also described. |
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AbstractList | Streptococcal pathogens cause a wide array of clinical syndromes in humans, including invasive systemic infections resulting in high mortality rates. Many of these pathogens are human specific, and therefore difficult to analyze in vivo using typical animal models, as these models rarely replicate what is observed in human infections. This unit describes the use of the zebrafish (Danio rerio) as an animal model for streptococcal infection to analyze multiple disease states. This model closely mimics the necrotizing fasciitis/myositis pathology observed in humans from a Streptococcus pyogenes infection. The use of a zoonotic pathogen, Streptococcus iniae, which replicates systemic infections caused by many streptococcal pathogens, including dissemination to the brain, is also described. Protocols describing both intraperitoneal and intramuscular infections, as well as methods for histological and quantitative measurements of infection, are also described. |
Author | Phelps, Hilary A Runft, Donna L Neely, Melody N |
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SubjectTerms | Animals Culture Techniques - methods Disease Models, Animal Humans Streptococcal Infections - microbiology Streptococcal Infections - pathology Streptococcus - pathogenicity Streptococcus - physiology Virulence Zebrafish - anatomy & histology Zebrafish - microbiology |
Title | Adult zebrafish model of streptococcal infection |
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