Adult zebrafish model of streptococcal infection

Streptococcal pathogens cause a wide array of clinical syndromes in humans, including invasive systemic infections resulting in high mortality rates. Many of these pathogens are human specific, and therefore difficult to analyze in vivo using typical animal models, as these models rarely replicate w...

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Bibliographic Details
Published inCurrent protocols in microbiology (Online) Vol. Chapter 9; p. Unit 9D.1
Main Authors Phelps, Hilary A, Runft, Donna L, Neely, Melody N
Format Journal Article
LanguageEnglish
Published United States 01.05.2009
Subjects
Animals
Culture Techniques - methods
Disease Models, Animal
Humans
Streptococcal Infections - microbiology
Streptococcal Infections - pathology
Streptococcus - pathogenicity
Streptococcus - physiology
Virulence
Zebrafish - anatomy & histology
Zebrafish - microbiology
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Summary:Streptococcal pathogens cause a wide array of clinical syndromes in humans, including invasive systemic infections resulting in high mortality rates. Many of these pathogens are human specific, and therefore difficult to analyze in vivo using typical animal models, as these models rarely replicate what is observed in human infections. This unit describes the use of the zebrafish (Danio rerio) as an animal model for streptococcal infection to analyze multiple disease states. This model closely mimics the necrotizing fasciitis/myositis pathology observed in humans from a Streptococcus pyogenes infection. The use of a zoonotic pathogen, Streptococcus iniae, which replicates systemic infections caused by many streptococcal pathogens, including dissemination to the brain, is also described. Protocols describing both intraperitoneal and intramuscular infections, as well as methods for histological and quantitative measurements of infection, are also described.
ISSN:1934-8533
DOI:10.1002/9780471729259.mc09d01s13