Clinical significance of serum miR-98-5p in children with Mycoplasma pneumoniae infection complicated with digestive system damage

• Published in: Immunobiology (1979) Vol. 230; no. 4; p. 153101
• Main Authors: Li, Ying, Jia, Wei, Huang, Jiayang, Liu, Jian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier GmbH 01.07.2025; Elsevier
• Subjects: Biomarkers - blood; Case-Control Studies; Child; Child, Preschool; Clinical Relevance; Diagnosis; Diarrhea; Diarrhea - blood; Diarrhea - diagnosis; Diarrhea - etiology; Female; Humans; Male; MicroRNAs - blood; MicroRNAs - genetics; miR-98-5p; Mycoplasma pneumoniae - physiology; Pneumonia, Mycoplasma - blood; Pneumonia, Mycoplasma - complications; Pneumonia, Mycoplasma - diagnosis; Pneumonia, Mycoplasma - genetics; ROC Curve; Diarrhea; MP; Diagnosis; miR-98-5p
• ISSN: 0171-2985; 1878-3279; 1878-3279
• DOI: 10.1016/j.imbio.2025.153101

Studies have shown that serum microRNA detection holds significant potential in improving the diagnostic accuracy of mycoplasma pneumoniae pneumonia (MPP). To investigate the expression and clinical value of miR-98-5p in children with Mycoplasma pneumoniae (MP) infection complicated with diarrhea. A total of 122 children diagnosed with MPP were selected as study subjects, and 80 healthy children were selected as controls. Real-time quantitative PCR (RT-qPCR) was used to detect the expression of miR-98-5p. Receiver operating characteristic (ROC) curve was used to determine the diagnostic value of miR-98-5p. The risk factors for MPP with diarrhea were analyzed by means of a logistic regression model. THP-1 cell models with miR-98-5p overexpression and knockdown were established to investigate the potential mechanisms of miR-98-5p in MP infection. Serum miR-98-5p expression is decreased among children having MPP, and it has a negative correlation with the logMP-DNA level. The miR-98-5p level in children with MPP accompanied by diarrhea is lower compared to that in children with MPP alone. miR-98-5p demonstrates promising diagnostic potential for MPP complicated with diarrhea, and serves as a risk factor for diarrhea in MP-infected children. TargetScan database prediction showed that potential target genes of miR-98-5p, including Synaptotagmin 1 (SYT1) and Tribbles pseudokinase 1(TRIB1), are both associated with digestive system injury induced by MP infection. The expression of serum miR-98-5p is decreased in children with MPP complicated by diarrhea, and it may become a diagnostic biomarker. •Serum miR-98-5p expression decreased in children with MPP complicated by diarrhea.•Serum miR-98-5p is correlated with logMP-DNA level in children with MPP.•Low miR-98-5p expression is a risk factor for MPP complicated by diarrhea.•miR-98-5p plays a role in MP infection by targeting SYT1.