The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks
Irvin A.D., Boarer C.D.H., Kurtti T.J. and Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks. International Journal for Parasitology 11:451–456. The uptake of radio-labelled nucl...
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Published in | International journal for parasitology Vol. 11; no. 6; pp. 451 - 456 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.01.1981
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Abstract | Irvin A.D.,
Boarer C.D.H.,
Kurtti T.J. and
Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by
Theileria parva in bovine blood and salivary glands of
Rhipicephalus appendiculatus ticks.
International Journal for Parasitology
11:451–456. The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of
Theileria pana was studied. Piroplasms took up tritiated purines, particularly hypoxanthine, but not pyrimidines. Similar uptake was recorded by
T. parva, both in tick saliva and in salivary glands maintained
in vitro. Intermediate parasite stages were those most readily labelled in glands; this reflected active nucleic acid synthesis associated with rapid parasite division. Radio-labelling of
T. parva in tick salivary glands could be of value in procedures used for concentrating and purifying theilerial sporozoites. |
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AbstractList | Irvin A.D.,
Boarer C.D.H.,
Kurtti T.J. and
Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by
Theileria parva in bovine blood and salivary glands of
Rhipicephalus appendiculatus ticks.
International Journal for Parasitology
11:451–456. The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of
Theileria pana was studied. Piroplasms took up tritiated purines, particularly hypoxanthine, but not pyrimidines. Similar uptake was recorded by
T. parva, both in tick saliva and in salivary glands maintained
in vitro. Intermediate parasite stages were those most readily labelled in glands; this reflected active nucleic acid synthesis associated with rapid parasite division. Radio-labelling of
T. parva in tick salivary glands could be of value in procedures used for concentrating and purifying theilerial sporozoites. The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of T. parva was studied. Piroplasms took up tritiated purines, particularly hypoxanthine, but not pyrimidines. Similar uptake was recorded by T. parva , both in tick saliva and in salivary glands maintained in vitro . Intermediate parasite stages were those most readily labelled in glands; this reflected active nucleic acid synthesis associated with rapid parasite division. Radio-labelling of T. parva in tick salivary glands could be of value in procedures used for concentrating and purifying theilerial sporozoites. |
Author | Irvin, A.D. Ocama, J.G.R. Boarer, C.D.H. Kurtti, T.J. |
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Keywords | tritium Theileria parva 14carbon tick piroplasm salivary gland hypoxanthine Rhipicephalus appendiculatus culture purine adenine thymidine sporozoites pyrimidine |
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References_xml | – volume: 19 start-page: 185 year: 1968 end-page: 197 ident: BIB1 article-title: Nukleinsaurenstoffwechsel bei experimenteller Malaria II publication-title: Zeitschrift für Tropenmedizin und Parasitologie contributor: fullname: Nielsen – start-page: 351 year: 1979 end-page: 371 ident: BIB10 article-title: Trends in tick cell culture publication-title: Practical Tissue Culture Applications contributor: fullname: Buscher – volume: 25 start-page: 227 year: 1974 end-page: 235 ident: BIB19 article-title: Hypoxanthine guanine phosphoribosyl transferase and adenine phosphoribosyl transferase from publication-title: Zeitschrift für Tropenmedizin und Parasitologie contributor: fullname: Konigk – volume: 82 start-page: 137 year: 1981 end-page: 147 ident: BIB4 article-title: Monitoring publication-title: Parasitology contributor: fullname: Ocama – volume: 56 start-page: 287 year: 1978 end-page: 303 ident: BIB17 article-title: Lichtmikroskopische Untersuchungen über die Entwicklung von publication-title: Zeitschrift für Parasitenkunde contributor: fullname: Friedhoff – volume: 1 start-page: 51 year: 1975 end-page: 60 ident: BIB16 article-title: East Coast fever; 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Snippet | Irvin A.D.,
Boarer C.D.H.,
Kurtti T.J. and
Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by
Theileria parva in bovine blood... The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of T. parva was studied. Piroplasms took up tritiated purines,... |
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SubjectTerms | 14carbon adenine Animals Apicomplexa - metabolism Cattle culture DNA Replication hypoxanthine piroplasm purine pyrimidine Rhipicephalus appendiculatus RNA - biosynthesis salivary gland Salivary Glands - metabolism sporozoites Theileria parva Theileriasis - blood thymidine tick Ticks - metabolism Tritium |
Title | The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks |
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