The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks

Irvin A.D., Boarer C.D.H., Kurtti T.J. and Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks. International Journal for Parasitology 11:451–456. The uptake of radio-labelled nucl...

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Published inInternational journal for parasitology Vol. 11; no. 6; pp. 451 - 456
Main Authors Irvin, A.D., Boarer, C.D.H., Kurtti, T.J., Ocama, J.G.R.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.01.1981
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Abstract Irvin A.D., Boarer C.D.H., Kurtti T.J. and Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks. International Journal for Parasitology 11:451–456. The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of Theileria pana was studied. Piroplasms took up tritiated purines, particularly hypoxanthine, but not pyrimidines. Similar uptake was recorded by T. parva, both in tick saliva and in salivary glands maintained in vitro. Intermediate parasite stages were those most readily labelled in glands; this reflected active nucleic acid synthesis associated with rapid parasite division. Radio-labelling of T. parva in tick salivary glands could be of value in procedures used for concentrating and purifying theilerial sporozoites.
AbstractList Irvin A.D., Boarer C.D.H., Kurtti T.J. and Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks. International Journal for Parasitology 11:451–456. The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of Theileria pana was studied. Piroplasms took up tritiated purines, particularly hypoxanthine, but not pyrimidines. Similar uptake was recorded by T. parva, both in tick saliva and in salivary glands maintained in vitro. Intermediate parasite stages were those most readily labelled in glands; this reflected active nucleic acid synthesis associated with rapid parasite division. Radio-labelling of T. parva in tick salivary glands could be of value in procedures used for concentrating and purifying theilerial sporozoites.
The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of T. parva was studied. Piroplasms took up tritiated purines, particularly hypoxanthine, but not pyrimidines. Similar uptake was recorded by T. parva , both in tick saliva and in salivary glands maintained in vitro . Intermediate parasite stages were those most readily labelled in glands; this reflected active nucleic acid synthesis associated with rapid parasite division. Radio-labelling of T. parva in tick salivary glands could be of value in procedures used for concentrating and purifying theilerial sporozoites.
Author Irvin, A.D.
Ocama, J.G.R.
Boarer, C.D.H.
Kurtti, T.J.
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Issue 6
Keywords tritium
Theileria parva
14carbon
tick
piroplasm
salivary gland
hypoxanthine
Rhipicephalus appendiculatus
culture
purine
adenine
thymidine
sporozoites
pyrimidine
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  ident: 10.1016/0020-7519(81)90063-1_BIB10
  article-title: Trends in tick cell culture
  contributor:
    fullname: Kurtti
– volume: 5
  start-page: 583
  year: 1973
  ident: 10.1016/0020-7519(81)90063-1_BIB2
  article-title: Cryopreservation of infective particles of Theileria parva
  publication-title: International Journal for Parasitology
  doi: 10.1016/0020-7519(73)90082-9
  contributor:
    fullname: Cunningham
– volume: 216
  start-page: 484
  year: 1967
  ident: 10.1016/0020-7519(81)90063-1_BIB13
  article-title: Artificial feeding technique for Rhipicephalus appendiculatus and the transmission of Theileria parva from the salivary secretion
  publication-title: Nature
  doi: 10.1038/216484a0
  contributor:
    fullname: Purnell
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Snippet Irvin A.D., Boarer C.D.H., Kurtti T.J. and Ocama J.G.R. 1981. The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood...
The uptake of radio-labelled nucleic acid precursors by blood and tick salivary gland forms of T. parva was studied. Piroplasms took up tritiated purines,...
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SubjectTerms 14carbon
adenine
Animals
Apicomplexa - metabolism
Cattle
culture
DNA Replication
hypoxanthine
piroplasm
purine
pyrimidine
Rhipicephalus appendiculatus
RNA - biosynthesis
salivary gland
Salivary Glands - metabolism
sporozoites
Theileria parva
Theileriasis - blood
thymidine
tick
Ticks - metabolism
Tritium
Title The incorporation of radio-labelled nucleic acid precursors by Theileria parva in bovine blood and salivary glands of Rhipicephalus appendiculatus ticks
URI https://dx.doi.org/10.1016/0020-7519(81)90063-1
https://www.ncbi.nlm.nih.gov/pubmed/6173342
https://search.proquest.com/docview/15376400
https://search.proquest.com/docview/73865846
Volume 11
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