The immunization protocol determines whether endogenous interferon-γ suppresses the infiltration of eosinophils into the conjunctiva
Studies with interferon-γ knockout (GKO) mice showed that endogenous IFN-γ suppresses the infiltration of inflammatory cells into the conjunctiva. To examine whether this phenomenon is universally true, we induced conjunctival inflammation by four different immunization protocols. Both wild type (WT...
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Published in | Immunology Letters Vol. 100; no. 2; pp. 189 - 194 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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15.09.2005
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Abstract | Studies with interferon-γ knockout (GKO) mice showed that endogenous IFN-γ suppresses the infiltration of inflammatory cells into the conjunctiva. To examine whether this phenomenon is universally true, we induced conjunctival inflammation by four different immunization protocols.
Both wild type (WT) and GKO mice (C57BL/6 background) were immunized with ragweed (RW) in aluminum hydroxide (alum). Two different immunization protocols were used: either the emulsion was injected into only the left hind footpad or it was also injected into the tail base (50
μg RW in 2
mg alum per injection site). In addition, to compare the effects of the immunization dose of RW and the immunization site, 100
μg RW in 2
mg alum was injected into only the left hind footpad and 25
μg RW in 2
mg alum per injection site was injected into both the left hind footpad and the tail base. Ten days later, the mice were challenged with 2
mg RW in 10
μl PBS. Twenty-four hours later, the conjunctivas were analyzed histologically, and the cellular and humoral immune responses in the spleens and sera were determined, respectively.
Similar to a previous report, GKO mice showed significant eosinophilic infiltration into the conjunctiva after the footpad only injection of 50
μg RW. However, injection of 50
μg RW per injection site into the footpad plus the tail base resulted in comparable levels of eosinophilic infiltration in WT and GKO mice. On the contrary, either immunization of 100
μg RW in 2
mg alum into only the left hind footpad or that of 25
μg RW in 2
mg alum into both the left hind footpad and the tail base induced significant infiltration of eosinophils into the conjunctiva of GKO mice, compared to WT mice.
These results show that the immunization protocol employed has a marked effect on the severity of eosinophilic infiltration. These observations indicate that in interpreting experimental results in the study of EC, the immunization protocol employed must be considered. |
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AbstractList | BACKGROUNDStudies with interferon-gamma knockout (GKO) mice showed that endogenous IFN-gamma suppresses the infiltration of inflammatory cells into the conjunctiva. To examine whether this phenomenon is universally true, we induced conjunctival inflammation by four different immunization protocols.METHODSBoth wild type (WT) and GKO mice (C57BL/6 background) were immunized with ragweed (RW) in aluminum hydroxide (alum). Two different immunization protocols were used: either the emulsion was injected into only the left hind footpad or it was also injected into the tail base (50 microg RW in 2mg alum per injection site). In addition, to compare the effects of the immunization dose of RW and the immunization site, 100 microg RW in 2mg alum was injected into only the left hind footpad and 25 microg RW in 2mg alum per injection site was injected into both the left hind footpad and the tail base. Ten days later, the mice were challenged with 2mg RW in 10 microl PBS. Twenty-four hours later, the conjunctivas were analyzed histologically, and the cellular and humoral immune responses in the spleens and sera were determined, respectively.RESULTSSimilar to a previous report, GKO mice showed significant eosinophilic infiltration into the conjunctiva after the footpad only injection of 50 microg RW. However, injection of 50 microg RW per injection site into the footpad plus the tail base resulted in comparable levels of eosinophilic infiltration in WT and GKO mice. On the contrary, either immunization of 100 microg RW in 2mg alum into only the left hind footpad or that of 25 microg RW in 2mg alum into both the left hind footpad and the tail base induced significant infiltration of eosinophils into the conjunctiva of GKO mice, compared to WT mice.CONCLUSIONSThese results show that the immunization protocol employed has a marked effect on the severity of eosinophilic infiltration. These observations indicate that in interpreting experimental results in the study of EC, the immunization protocol employed must be considered. Background:: Studies with interferon- gamma knockout (GKO) mice showed that endogenous IFN- gamma suppresses the infiltration of inflammatory cells into the conjunctiva. To examine whether this phenomenon is universally true, we induced conjunctival inflammation by four different immunization protocols. Methods:: Both wild type (WT) and GKO mice (C57BL/6 background) were immunized with ragweed (RW) in aluminum hydroxide (alum). Two different immunization protocols were used: either the emulsion was injected into only the left hind footpad or it was also injected into the tail base (50 mu g RW in 2mg alum per injection site). In addition, to compare the effects of the immunization dose of RW and the immunization site, 100 mu g RW in 2mg alum was injected into only the left hind footpad and 25 mu g RW in 2mg alum per injection site was injected into both the left hind footpad and the tail base. Ten days later, the mice were challenged with 2mg RW in 10 mu l PBS. Twenty-four hours later, the conjunctivas were analyzed histologically, and the cellular and humoral immune responses in the spleens and sera were determined, respectively. Results:: Similar to a previous report, GKO mice showed significant eosinophilic infiltration into the conjunctiva after the footpad only injection of 50 mu g RW. However, injection of 50 mu g RW per injection site into the footpad plus the tail base resulted in comparable levels of eosinophilic infiltration in WT and GKO mice. On the contrary, either immunization of 100 mu g RW in 2mg alum into only the left hind footpad or that of 25 mu g RW in 2mg alum into both the left hind footpad and the tail base induced significant infiltration of eosinophils into the conjunctiva of GKO mice, compared to WT mice. Conclusions:: These results show that the immunization protocol employed has a marked effect on the severity of eosinophilic infiltration. These observations indicate that in interpreting experimental results in the study of EC, the immunization protocol employed must be considered. Studies with interferon-γ knockout (GKO) mice showed that endogenous IFN-γ suppresses the infiltration of inflammatory cells into the conjunctiva. To examine whether this phenomenon is universally true, we induced conjunctival inflammation by four different immunization protocols. Both wild type (WT) and GKO mice (C57BL/6 background) were immunized with ragweed (RW) in aluminum hydroxide (alum). Two different immunization protocols were used: either the emulsion was injected into only the left hind footpad or it was also injected into the tail base (50 μg RW in 2 mg alum per injection site). In addition, to compare the effects of the immunization dose of RW and the immunization site, 100 μg RW in 2 mg alum was injected into only the left hind footpad and 25 μg RW in 2 mg alum per injection site was injected into both the left hind footpad and the tail base. Ten days later, the mice were challenged with 2 mg RW in 10 μl PBS. Twenty-four hours later, the conjunctivas were analyzed histologically, and the cellular and humoral immune responses in the spleens and sera were determined, respectively. Similar to a previous report, GKO mice showed significant eosinophilic infiltration into the conjunctiva after the footpad only injection of 50 μg RW. However, injection of 50 μg RW per injection site into the footpad plus the tail base resulted in comparable levels of eosinophilic infiltration in WT and GKO mice. On the contrary, either immunization of 100 μg RW in 2 mg alum into only the left hind footpad or that of 25 μg RW in 2 mg alum into both the left hind footpad and the tail base induced significant infiltration of eosinophils into the conjunctiva of GKO mice, compared to WT mice. These results show that the immunization protocol employed has a marked effect on the severity of eosinophilic infiltration. These observations indicate that in interpreting experimental results in the study of EC, the immunization protocol employed must be considered. Studies with interferon-gamma knockout (GKO) mice showed that endogenous IFN-gamma suppresses the infiltration of inflammatory cells into the conjunctiva. To examine whether this phenomenon is universally true, we induced conjunctival inflammation by four different immunization protocols. Both wild type (WT) and GKO mice (C57BL/6 background) were immunized with ragweed (RW) in aluminum hydroxide (alum). Two different immunization protocols were used: either the emulsion was injected into only the left hind footpad or it was also injected into the tail base (50 microg RW in 2mg alum per injection site). In addition, to compare the effects of the immunization dose of RW and the immunization site, 100 microg RW in 2mg alum was injected into only the left hind footpad and 25 microg RW in 2mg alum per injection site was injected into both the left hind footpad and the tail base. Ten days later, the mice were challenged with 2mg RW in 10 microl PBS. Twenty-four hours later, the conjunctivas were analyzed histologically, and the cellular and humoral immune responses in the spleens and sera were determined, respectively. Similar to a previous report, GKO mice showed significant eosinophilic infiltration into the conjunctiva after the footpad only injection of 50 microg RW. However, injection of 50 microg RW per injection site into the footpad plus the tail base resulted in comparable levels of eosinophilic infiltration in WT and GKO mice. On the contrary, either immunization of 100 microg RW in 2mg alum into only the left hind footpad or that of 25 microg RW in 2mg alum into both the left hind footpad and the tail base induced significant infiltration of eosinophils into the conjunctiva of GKO mice, compared to WT mice. These results show that the immunization protocol employed has a marked effect on the severity of eosinophilic infiltration. These observations indicate that in interpreting experimental results in the study of EC, the immunization protocol employed must be considered. |
Author | Fukushima, Atsuki Ishida, Waka Ozaki, Akemi Yamaguchi, Tomoko Fukata, Kazuyo Ueno, Hisayuki |
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Keywords | Interferon-gamma Allergic conjunctivitis Knockout mouse Active immunization |
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References | Magone, Whitcup, Fukushima, Chan, Silver, Rizzo (bib5) 2000; 105 Fukushima, Ozaki, Jian, Ishida, Fukata, Liu, Ueno (bib8) 2005; 30 Magone, Chan, Rizzo, Kozhich, Whitcup (bib4) 1998; 87 Groneberg, Bielory, Fischer, Bonini, Wahn (bib3) 2003; 58 Dalton, Pitts-Meek, Keshav, Figari, Bradley, Stewart (bib7) 1993; 259 Agarwal, Caspi (bib9) 2004; 102 Fukushima, Fukata, Ozaki, Takata, Kuroda, Enzan, Ueno (bib6) 2002; 86 Wekerle, Kojima, Lannes-Vieira, Lassmann, Linington (bib1) 1994; 36 Fox, Kuwabara, Wiggert, Redmond, Hess, Chader, Gery (bib2) 1987; 43 Magone (10.1016/j.imlet.2005.04.012_bib5) 2000; 105 Fox (10.1016/j.imlet.2005.04.012_bib2) 1987; 43 Fukushima (10.1016/j.imlet.2005.04.012_bib6) 2002; 86 Wekerle (10.1016/j.imlet.2005.04.012_bib1) 1994; 36 Magone (10.1016/j.imlet.2005.04.012_bib4) 1998; 87 Fukushima (10.1016/j.imlet.2005.04.012_bib8) 2005; 30 Agarwal (10.1016/j.imlet.2005.04.012_bib9) 2004; 102 Groneberg (10.1016/j.imlet.2005.04.012_bib3) 2003; 58 Dalton (10.1016/j.imlet.2005.04.012_bib7) 1993; 259 |
References_xml | – volume: 86 start-page: 1166 year: 2002 end-page: 1171 ident: bib6 article-title: Exertion of the suppressive effects of IFN-gamma on experimental immune mediated blepharoconjunctivitis in Brown Norway rats during the induction phase but not the effector phase publication-title: Br J Ophthalmol contributor: fullname: Ueno – volume: 58 start-page: 1101 year: 2003 end-page: 1113 ident: bib3 article-title: Animal models of allergic and inflammatory conjunctivitis publication-title: Allergy contributor: fullname: Wahn – volume: 102 start-page: 395 year: 2004 end-page: 420 ident: bib9 article-title: Rodent models of experimental autoimmune uveitis publication-title: Meth Mol Med contributor: fullname: Caspi – volume: 43 start-page: 256 year: 1987 end-page: 264 ident: bib2 article-title: Experimental autoimmune uveoretinitis (EAU) induced by retinal interphotoreceptor retinoid-binding protein (IRBP): differences between EAU induced by IRBP and by S-antigen publication-title: Clin Immunol Immunopathol contributor: fullname: Gery – volume: 30 start-page: 241 year: 2005 end-page: 248 ident: bib8 article-title: Dissection of antigen-specific humoral and cellular immune responses for the development of experimental immune-mediated blepharoconjunctivitis (EC) in C57BL/6 mice publication-title: Curr Eye Res contributor: fullname: Ueno – volume: 259 start-page: 1739 year: 1993 end-page: 1742 ident: bib7 article-title: Multiple defects of immune cell function in mice with disrupted interferon-gamma genes publication-title: Science contributor: fullname: Stewart – volume: 36 start-page: 47 year: 1994 end-page: 53 ident: bib1 article-title: Animal models publication-title: Ann Neurol contributor: fullname: Linington – volume: 105 start-page: 299 year: 2000 end-page: 308 ident: bib5 article-title: The role of IL-12 in the induction of late-phase cellular infiltration in a murine model of allergic conjunctivitis publication-title: J Allergy Clin Immunol contributor: fullname: Rizzo – volume: 87 start-page: 75 year: 1998 end-page: 84 ident: bib4 article-title: A novel murine model of allergic conjunctivitis publication-title: Clin Immunol Immunopathol contributor: fullname: Whitcup – volume: 259 start-page: 1739 year: 1993 ident: 10.1016/j.imlet.2005.04.012_bib7 article-title: Multiple defects of immune cell function in mice with disrupted interferon-gamma genes publication-title: Science doi: 10.1126/science.8456300 contributor: fullname: Dalton – volume: 87 start-page: 75 year: 1998 ident: 10.1016/j.imlet.2005.04.012_bib4 article-title: A novel murine model of allergic conjunctivitis publication-title: Clin Immunol Immunopathol doi: 10.1006/clin.1997.4507 contributor: fullname: Magone – volume: 58 start-page: 1101 year: 2003 ident: 10.1016/j.imlet.2005.04.012_bib3 article-title: Animal models of allergic and inflammatory conjunctivitis publication-title: Allergy doi: 10.1046/j.1398-9995.2003.00326.x contributor: fullname: Groneberg – volume: 105 start-page: 299 year: 2000 ident: 10.1016/j.imlet.2005.04.012_bib5 article-title: The role of IL-12 in the induction of late-phase cellular infiltration in a murine model of allergic conjunctivitis publication-title: J Allergy Clin Immunol doi: 10.1016/S0091-6749(00)90080-0 contributor: fullname: Magone – volume: 86 start-page: 1166 year: 2002 ident: 10.1016/j.imlet.2005.04.012_bib6 article-title: Exertion of the suppressive effects of IFN-gamma on experimental immune mediated blepharoconjunctivitis in Brown Norway rats during the induction phase but not the effector phase publication-title: Br J Ophthalmol doi: 10.1136/bjo.86.10.1166 contributor: fullname: Fukushima – volume: 36 start-page: 47 issue: Suppl. year: 1994 ident: 10.1016/j.imlet.2005.04.012_bib1 article-title: Animal models publication-title: Ann Neurol doi: 10.1002/ana.410360714 contributor: fullname: Wekerle – volume: 43 start-page: 256 year: 1987 ident: 10.1016/j.imlet.2005.04.012_bib2 article-title: Experimental autoimmune uveoretinitis (EAU) induced by retinal interphotoreceptor retinoid-binding protein (IRBP): differences between EAU induced by IRBP and by S-antigen publication-title: Clin Immunol Immunopathol doi: 10.1016/0090-1229(87)90133-4 contributor: fullname: Fox – volume: 30 start-page: 241 year: 2005 ident: 10.1016/j.imlet.2005.04.012_bib8 article-title: Dissection of antigen-specific humoral and cellular immune responses for the development of experimental immune-mediated blepharoconjunctivitis (EC) in C57BL/6 mice publication-title: Curr Eye Res doi: 10.1080/02713680590927560 contributor: fullname: Fukushima – volume: 102 start-page: 395 year: 2004 ident: 10.1016/j.imlet.2005.04.012_bib9 article-title: Rodent models of experimental autoimmune uveitis publication-title: Meth Mol Med contributor: fullname: Agarwal |
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Snippet | Studies with interferon-γ knockout (GKO) mice showed that endogenous IFN-γ suppresses the infiltration of inflammatory cells into the conjunctiva. To examine... Studies with interferon-gamma knockout (GKO) mice showed that endogenous IFN-gamma suppresses the infiltration of inflammatory cells into the conjunctiva. To... Background:: Studies with interferon- gamma knockout (GKO) mice showed that endogenous IFN- gamma suppresses the infiltration of inflammatory cells into the... BACKGROUNDStudies with interferon-gamma knockout (GKO) mice showed that endogenous IFN-gamma suppresses the infiltration of inflammatory cells into the... |
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SubjectTerms | Active immunization Allergens - immunology Allergic conjunctivitis Ambrosia Animals Cell Proliferation Cells, Cultured Conjunctivitis, Allergic - etiology Conjunctivitis, Allergic - immunology Conjunctivitis, Allergic - pathology Dose-Response Relationship, Immunologic Eosinophils - immunology Immunization - methods Immunoglobulin E - blood Interferon-gamma Interferon-gamma - genetics Interferon-gamma - immunology Interleukin-4 - biosynthesis Knockout mouse Mice Mice, Inbred C57BL Mice, Knockout Pollen - immunology Spleen - cytology Spleen - immunology Spleen - metabolism |
Title | The immunization protocol determines whether endogenous interferon-γ suppresses the infiltration of eosinophils into the conjunctiva |
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