Delta-endotoxins form cation-selective channels in planar lipid bilayers

• Published in: Biochemical and biophysical research communications Vol. 169; no. 2; pp. 765 - 772
• Main Authors: Slatin, Stephen L., Abrams, Charles K., English, Leigh
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.06.1990
• Subjects: Bacillus thuringiensis; Bacterial Proteins; Bacterial Toxins; calcium; Cholesterol; Electrophoresis, Polyacrylamide Gel; endotoxins; Endotoxins - isolation & purification; Hemolysin Proteins; Ion Channels; Lipid Bilayers; Membrane Potentials; Phosphatidylcholines; Phospholipids

Delta-endotoxins CryIA(c) and CryIIIA, two members of a large family of toxic proteins from Bacillus thuringiensis, were each allowed to interact with planar lipid bilayers and were analyzed for their ability to form ion-conducting channels. Both of these toxins made clearly resolved channels in the...