EROD activities in a primary cell culture of grass carp ( Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas
Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin- O-deethylase (EROD) assay of a primary hepatocyte culture from grass carp ( Ctenopharyngodon idellus). The results were compared wit...
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Published in | Ecotoxicology and environmental safety Vol. 58; no. 1; pp. 84 - 89 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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01.05.2004
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Abstract | Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin-
O-deethylase (EROD) assay of a primary hepatocyte culture from grass carp (
Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose–response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo-
p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC
50=0.83
pg/mL) is more sensitive to TCDD than the assay based on primary hepatocyte culture (EC
50=9.7
pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo-
p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione-
S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. |
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AbstractList | Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin-O-deethylase (EROD) assay of a primary hepatocyte culture from grass carp (Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose-response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC50=0.83 pg/mL) is more sensitive to TCDD than the assay based on primary hepatocyte culture (EC50=9.7 pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin- O-deethylase (EROD) assay of a primary hepatocyte culture from grass carp ( Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose–response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC 50=0.83 pg/mL) is more sensitive to TCDD than the assay based on primary hepatocyte culture (EC 50=9.7 pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo- p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione- S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. |
Author | Wan, Xiaoqiong Ma, Taowu Wang, Zijian Wu, Wenzhong |
Author_xml | – sequence: 1 givenname: Xiaoqiong surname: Wan fullname: Wan, Xiaoqiong email: wangzj@mail.rceees.ac.cn organization: Research Center for Eco-environmental Sciences, State Key Laboratory of Environmental Aquatic Chemistry, Chinese Academy of Sciences, P.O. Box. 2871, Beijing 100085, China – sequence: 2 givenname: Taowu surname: Ma fullname: Ma, Taowu organization: Research Center for Eco-environmental Sciences, State Key Laboratory of Environmental Aquatic Chemistry, Chinese Academy of Sciences, P.O. Box. 2871, Beijing 100085, China – sequence: 3 givenname: Wenzhong surname: Wu fullname: Wu, Wenzhong organization: Institute of Hydrobiology, State Key Laboratory for Freshwater Ecology and Biotechnology, Chinese Academy of Sciences, Wuhan 430072, China – sequence: 4 givenname: Zijian surname: Wang fullname: Wang, Zijian organization: Research Center for Eco-environmental Sciences, State Key Laboratory of Environmental Aquatic Chemistry, Chinese Academy of Sciences, P.O. Box. 2871, Beijing 100085, China |
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Cites_doi | 10.1016/0003-2697(76)90527-3 10.1139/f85-222 10.1016/0141-1136(92)90088-4 10.1016/S0166-445X(99)00025-9 10.1016/0041-008X(90)90105-4 10.1007/BF02631451 10.1007/BF02624453 10.1016/S0076-6879(81)77053-8 10.1242/jeb.175.1.219 10.1016/S0166-445X(96)00811-9 10.1016/0014-5793(86)80174-0 10.1007/BF01378789 10.1139/f94-156 10.1016/0009-8981(73)90475-0 10.1016/0016-6480(88)90148-7 10.1146/annurev.pa.22.040182.002505 10.1006/taap.1999.8663 10.1016/S0166-445X(99)00008-9 10.1016/0003-9861(92)90072-5 |
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Keywords | Primary hepatocyte culture EROD Grass carp ( Ctenopharyngodon idellus) Polychlorinated aromatic hydrocarbons Cell culture Ctenopharyngodon idella Ecotoxicology Digestive system Toxicity Liver Polycyclic aromatic compound Ah receptor Vertebrata Enzymatic activity Hepatocyte Pisces Environment Aromatic compound Cyprinidae Biological effect Grass carp (Ctenopharyngodon idellus) Organic compounds |
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SubjectTerms | Animal, plant and microbial ecology Animals Applied ecology Biodegradation, Environmental Biological and medical sciences Carps - physiology Cell Culture Techniques Cytochrome P-450 CYP1A1 - pharmacology Ecotoxicology, biological effects of pollution Environmental Pollutants - metabolism Environmental Pollutants - toxicity EROD Fundamental and applied biological sciences. Psychology General aspects Glutathione Transferase - pharmacology Grass carp ( Ctenopharyngodon idellus) Hepatocytes - drug effects Hepatocytes - physiology Male Oxidoreductases - pharmacology Polychlorinated aromatic hydrocarbons Polychlorinated Dibenzodioxins - metabolism Polychlorinated Dibenzodioxins - toxicity Primary hepatocyte culture |
Title | EROD activities in a primary cell culture of grass carp ( Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas |
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