Standardized method for the determination of human erythrocyte membrane adenosine triphosphatases

• Published in: Analytical biochemistry Vol. 124; no. 1; pp. 19 - 26
• Main Authors: Reinila, Matti, MacDonald, Ewen, Salem, Norman, Linnoila, Markku, Trams, Eberhard G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.07.1982
• Subjects: Adenosine Triphosphatases - blood; Adenosine Triphosphatases - isolation & purification; adenosinetriphosphatase; assays; cell membranes; Chromatography, High Pressure Liquid; Colorimetry; Erythrocyte Membrane - enzymology; erythrocytes; Erythrocytes - enzymology; Humans; Kinetics; man; Phosphorus Radioisotopes

A standardized assay is described for the simultaneous determination of Mg 2+-ATPase, Na +, K +-ATPase, and Ca 2+-ATPase in human erythrocyte (RBC) membrane preparations. Membranes were prepared by lysis of RBCs in hypotonic buffer, and ATPase activity assays were based on the measurement of 32P-lab...