Free Radical Scavenging Activity and Secondary Metabolites from in vitro Cultures of Sanicula graveolens

• Published in: Zeitschrift für Naturforschung C. A journal of biosciences Vol. 62; no. 7; pp. 555 - 562
• Main Authors: Cheel, José, Schmeda-Hirschmann, Guillermo, Jordan, Miguel, Theoduloz, Cristina, Rodríguez, Jaime A., Gerth, André, Wilken, Dirk
• Format: Journal Article
• Language: English
• Published: Germany Verlag der Zeitschrift für Naturforschung 01.07.2007
• Subjects: Biphenyl Compounds; Chromatography, High Pressure Liquid; Free Radical Scavengers - isolation & purification; Free Radical Scavengers - pharmacology; Freeze Drying; Hydrazines; In vitro Propagation; Picrates; Plant Growth Regulators - pharmacology; Plant Leaves - chemistry; Plant Leaves - physiology; Plant Shoots - chemistry; Plant Shoots - physiology; Plant Stems - chemistry; Plant Stems - physiology; Plant Structures - chemistry; Sanicula - chemistry; Sanicula - drug effects; Sanicula - physiology; Sanicula graveolens; Secondary Metabolite Content; Spices
• DOI: 10.1515/znc-2007-7-815
• ISSN: 0939-5075

An in vitro propagation system was developed to obtain shoot and root cultures from the Andean spice Sanicula graveolens (Apiaceae). Propagation of shoots, roots and plantlets was achieved by the temporary immersion system. The free radical scavenging effect of the methanol/ water (7 :3 v/v) extracts was determined by the discoloration of the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Total phenolic, flavonoid, chlorogenic acid (CA) and quercetin 3-O-glucoside content in the samples was assessed by spectrophotometry and DAD-HPLC analysis, respectively. On a dry weight basis, the crude extracts showed total phenolic values ranging from 3.57 to 6.93%, with highest content for the root culture sample. Total flavonoid content ranged from 1.23 to 2.23% and was lower for the root culture. Chlorogenic acid and neochlorogenic acid were identified by TLC in all samples. Highest free radical scavenging effect was observed for the root culture which also presented the highest CA content. Two of the shoot culture samples, with similar IC values in the DPPH discoloration assay, also presented close quercetin-3-O-glucoside content