Evidence for the association of I-A and I-E molecules in d-haplotype mice
Previous studies using sequential immunoprecipitation indicated the existence of two I-E molecules in d-haplotype mice. Using N-terminal amino acid radiosequence analysis, we have shown that the sequence of the alpha- and beta-chains from the I-E molecule, which is immunoprecipitated by a monoclonal...
Saved in:
Published in | Molecular immunology Vol. 23; no. 3; p. 263 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
01.03.1986
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Previous studies using sequential immunoprecipitation indicated the existence of two I-E molecules in d-haplotype mice. Using N-terminal amino acid radiosequence analysis, we have shown that the sequence of the alpha- and beta-chains from the I-E molecule, which is immunoprecipitated by a monoclonal antibody (14.4.4S), is consistent with that determined for the gene of the I-Ed molecule. The material, which is immunoprecipitated by an anti-I-Ed alloantiserum [(B10 X D2.GD)F1 anti-B10.D2] after preclearing with 14.4.4S, has a N-terminal amino acid sequence consistent with it being a mixture of the I-Ed and I-Ad alpha- and beta-chains. The amount of I-Ad varied with each preparation, ranging from 15 to 40% of the total. Nonequilibrium pH gradient gel electrophoresis fractionation of the beta-chain pool precipitated by the alloantiserum yielded two molecules. One molecule had an Ad beta N-terminal amino acid sequence and the other had an Ed beta N-terminal sequence. The existence of antibodies cross-reactive with I-A in the anti-I-E alloantiserum were ruled out because this antiserum could not immunoprecipitate any I-Ad molecules from a D2.GD spleen cell lysate. Therefore, we conclude that the alloantiserum is recognizing determinants that are formed by an interaction between I-Ad and I-Ed molecules or their subunits. |
---|---|
AbstractList | Previous studies using sequential immunoprecipitation indicated the existence of two I-E molecules in d-haplotype mice. Using N-terminal amino acid radiosequence analysis, we have shown that the sequence of the alpha- and beta-chains from the I-E molecule, which is immunoprecipitated by a monoclonal antibody (14.4.4S), is consistent with that determined for the gene of the I-Ed molecule. The material, which is immunoprecipitated by an anti-I-Ed alloantiserum [(B10 X D2.GD)F1 anti-B10.D2] after preclearing with 14.4.4S, has a N-terminal amino acid sequence consistent with it being a mixture of the I-Ed and I-Ad alpha- and beta-chains. The amount of I-Ad varied with each preparation, ranging from 15 to 40% of the total. Nonequilibrium pH gradient gel electrophoresis fractionation of the beta-chain pool precipitated by the alloantiserum yielded two molecules. One molecule had an Ad beta N-terminal amino acid sequence and the other had an Ed beta N-terminal sequence. The existence of antibodies cross-reactive with I-A in the anti-I-E alloantiserum were ruled out because this antiserum could not immunoprecipitate any I-Ad molecules from a D2.GD spleen cell lysate. Therefore, we conclude that the alloantiserum is recognizing determinants that are formed by an interaction between I-Ad and I-Ed molecules or their subunits. |
Author | Maloy, W L Sachs, D H Coligan, J E Ozato, K |
Author_xml | – sequence: 1 givenname: W L surname: Maloy fullname: Maloy, W L – sequence: 2 givenname: K surname: Ozato fullname: Ozato, K – sequence: 3 givenname: D H surname: Sachs fullname: Sachs, D H – sequence: 4 givenname: J E surname: Coligan fullname: Coligan, J E |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/3459016$$D View this record in MEDLINE/PubMed |
BookMark | eNo9j01Lw0AYhPdQqW31HyjsUQ-rbza7m82xlFQDBS96Lm_2g64k2ZBNhf57AxYPwwMzMMysyaKPvSPkIYOXDDL1OitjUpfwpNVzCSA5gwVZ_du3ZJ3SNwAoUHJJlrmQ5RyuSF39BOt646iPI51OjmJK0QScQuxp9LRmW4q9nVnRLrbOnFuXaOipZScc2jhdBke7YNwdufHYJnd_5YZ87avP3Ts7fLzVu-2BmVzriaGwXOXKZ9LmjoMQBrH0IB1CqYWGpoDCWgl2HioK5AiNbUqvczCAXiu-IY9_vcO56Zw9DmPocLwcr5f4L05kTLA |
CitedBy_id | crossref_primary_10_1016_0092_8674_89_90794_0 crossref_primary_10_1038_329339a0 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1016/0161-5890(86)90052-0 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine Chemistry Biology |
ExternalDocumentID | 3459016 |
Genre | Journal Article |
GroupedDBID | --- --K --M -~X .55 .GJ .~1 0R~ 123 1B1 1RT 1~. 1~5 29M 3O- 4.4 457 4G. 53G 5RE 5VS 7-5 71M 8P~ 9JM AAAJQ AABNK AACTN AAEDT AAEDW AAHBH AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXKI AAXUO ABBQC ABEFU ABFNM ABFRF ABGSF ABJNI ABMAC ABMZM ABOCM ABUDA ABXDB ACDAQ ACGFO ACGFS ACIUM ACNCT ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFFNX AFJKZ AFKWA AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJOXV AJRQY AKRWK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV C45 CGR CJTIS CNWQP CS3 CUY CVF DU5 EBS ECM EFJIC EIF EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HMG HVGLF HZ~ H~9 IH2 IHE J1W K-O KOM L7B LUGTX M41 MO0 N9A NPM O-L O9- OAUVE OVD OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SDF SDG SDP SES SEW SIN SPCBC SSH SSI SSU SSZ T5K TEORI UNMZH WUQ X7M Y6R ZGI ~G- |
ID | FETCH-LOGICAL-c388t-a4d2636f15d3e2044caa9f05ea098480b707dd50d06547a2a0bdb9f830c0af862 |
ISSN | 0161-5890 |
IngestDate | Sat Sep 28 08:41:07 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 3 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c388t-a4d2636f15d3e2044caa9f05ea098480b707dd50d06547a2a0bdb9f830c0af862 |
PMID | 3459016 |
ParticipantIDs | pubmed_primary_3459016 |
PublicationCentury | 1900 |
PublicationDate | 1986-03-01 |
PublicationDateYYYYMMDD | 1986-03-01 |
PublicationDate_xml | – month: 03 year: 1986 text: 1986-03-01 day: 01 |
PublicationDecade | 1980 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Molecular immunology |
PublicationTitleAlternate | Mol Immunol |
PublicationYear | 1986 |
SSID | ssj0006065 |
Score | 1.3466138 |
Snippet | Previous studies using sequential immunoprecipitation indicated the existence of two I-E molecules in d-haplotype mice. Using N-terminal amino acid... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 263 |
SubjectTerms | Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antigen-Antibody Reactions Cross Reactions Electrophoresis Electrophoresis, Polyacrylamide Gel Haploidy Histocompatibility Antigens Class II - analysis Histocompatibility Antigens Class II - immunology Immune Sera - immunology Major Histocompatibility Complex Mice Mice, Inbred Strains |
Title | Evidence for the association of I-A and I-E molecules in d-haplotype mice |
URI | https://www.ncbi.nlm.nih.gov/pubmed/3459016 |
Volume | 23 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwELYKiMeCoFDxlgcGUGVwHMdxR1QVAVJhAcEEcmIHKvU1lAF-PefYScNLPJYkshXL8n05313u8yG0TwPNaNxiRGjFCOwQkiSpDAlV4G4FTMsksnzn7qU4u-EXd9FdrXZfyVp6niRH6euXvJL_SBXaQK6WJfsHyZaDQgM8g3zhChKG669kXJQELXMF1XSxc44IOXH5vqTTHLg6uMblv5InNe6P8vjrwGe_FSZqtyiY2-xZ7si7sHtX9V3s7rZZBo2vXlVeiqkSMLVnlOfKbEp9aI_6vUcXbL3w7AftyHdSTJOtivCjCEgkXYHPQn86vrDHSVhVhk51fVLSLl5QjgWWtP2JBNsijRih1VdguceDXHghtyRZ8WPnh8Ozfc8Mmoml1X-XNpbj92lw3VyCq59HQawMxHHZdiDFoZ_XEpr3o33wQHJL5HoFLXsXAp84PKyimhnW0bwrKvpSR4vtooZfHS10ferEGjovwIIBLBjAgitgwaMMA1gwgAXuHVyCBfeGuAIWbMGyjm5OO9ftM-LraJA0lHJCFNcgCpEFkQ4No5ynSrUyGhlFW5JLmsQ01jqi2hKNY8UUTXTSymRIU6oycHkbaHY4GpoNhOETDgKjAq654sb-kmeCxVQnJgNDMUo3UcMtzcPYHZby4Nds67uObbQ0RdoOmsvg2zS7YOhNkr1cXm_ZPEf5 |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Evidence+for+the+association+of+I-A+and+I-E+molecules+in+d-haplotype+mice&rft.jtitle=Molecular+immunology&rft.au=Maloy%2C+W+L&rft.au=Ozato%2C+K&rft.au=Sachs%2C+D+H&rft.au=Coligan%2C+J+E&rft.date=1986-03-01&rft.issn=0161-5890&rft.volume=23&rft.issue=3&rft.spage=263&rft_id=info:doi/10.1016%2F0161-5890%2886%2990052-0&rft_id=info%3Apmid%2F3459016&rft_id=info%3Apmid%2F3459016&rft.externalDocID=3459016 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0161-5890&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0161-5890&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0161-5890&client=summon |