Development of A Standardized Opsonophagocytosis Killing Assay for Group B Streptococcus and Assessment in an Interlaboratory Study

• Published in: Vaccines (Basel) Vol. 11; no. 11; p. 1703
• Main Authors: Leung, Stephanie, Collett, Clare F, Allen, Lauren, Lim, Suzanna, Maniatis, Pete, Bolcen, Shanna J, Alston, Bailey, Patel, Palak Y, Kwatra, Gaurav, Hall, Tom, Thomas, Stephen, Taylor, Stephen, Le Doare, Kirsty, Gorringe, Andrew
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.11.2023
• Subjects: Animal vaccines; Antibodies; Assaying; Bacteria; Capsular polysaccharides; Cell differentiation; Consortia; correlates of protection; Flow cytometry; Group B Streptococcus; Immune clearance; Immunoassay; Immunoglobulin G; Licenses; Medical schools; Medicine; Monoclonal antibodies; neonatal antibodies; OPKA; Opsonophagocytosis; opsonophagocytosis assay; Phagocytes; Placental transfer; Polysaccharides; R&D; Reagents; Research & development; Standardization; Streptococcus; Streptococcus infections; Vaccine development; Vaccines; Denmark; Canada; United Kingdom > UK; United States > US; correlates of protection; OPKA; vaccines; Group B Streptococcus; opsonophagocytosis assay; transplacental antibodies; neonatal antibodies

The placental transfer of antibodies that mediate bacterial clearance via phagocytes is likely important for protection against invasive group B (GBS) disease. A robust functional assay is essential to determine the immune correlates of protection and assist vaccine development. Using standard reage...