Effect of trimming inhibitors on the secretion and biological activity of a murine IgE monoclonal antibody

• Published in: Molecular immunology Vol. 24; no. 8; p. 849
• Main Authors: Granato, D A, Neeser, J R
• Format: Journal Article
• Language: English
• Published: England 01.08.1987
• Subjects: 1-Deoxynojirimycin - analogs & derivatives; Alkaloids - pharmacology; Animals; Antibodies, Monoclonal - biosynthesis; Cell Line; Glucosamine - analogs & derivatives; Glucosamine - pharmacology; Glycosylation; Immunoglobulin E - biosynthesis; Immunoglobulin E - metabolism; Indolizines; Mice; Protein Processing, Post-Translational - drug effects; Swainsonine; Tunicamycin - pharmacology

Since secretion of IgE antibodies is known to be blocked by tunicamycin, the first aim of the present study was to determine at which step of glycosylation or processing secretion was restored. For this purpose, murine hybridoma cells secreting an anti beta-lactoglobulin IgE were incubated either in...