Differential Mitochondrial DNA and Gene Expression in Inherited Retinal Dysplasia in Miniature Schnauzer Dogs

• Published in: Investigative ophthalmology & visual science Vol. 47; no. 5; pp. 1810 - 1816
• Main Authors: Appleyard, Greg D, Forsyth, George W, Kiehlbauch, Laura M, Sigfrid, Kristen N, Hanik, Heather L. J, Quon, Anita, Loewen, Matthew E, Grahn, Bruce H
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.05.2006; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Biological and medical sciences; DNA, Complementary - genetics; DNA, Mitochondrial - genetics; Dog Diseases - genetics; Dog Diseases - pathology; Dogs; Electron Transport Complex IV - genetics; Eye and associated structures. Visual pathways and centers. Vision; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) - genetics; Male; Malformations of the eye; Medical sciences; Mitochondria, Muscle - genetics; Mitochondria, Muscle - ultrastructure; NADH Dehydrogenase - genetics; Oligonucleotide Array Sequence Analysis - veterinary; Ophthalmology; Pigment Epithelium of Eye - metabolism; Retina - metabolism; Retinal Dysplasia - genetics; Retinal Dysplasia - pathology; Retinal Dysplasia - veterinary; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA - isolation & purification; RNA, Messenger - analysis; Vertebrates: nervous system and sense organs; Fissipedia; Retinal dysplasia; Carnivora; Mitochondrial DNA; Gene expression; Genetic disease; Eye disease; Vertebrata; Mammalia; Malformation; Animal; Ophthalmology; Dog

To investigate the molecular basis of inherited retinal dysplasia in miniature Schnauzers. Retina and retinal pigment epithelial tissues were collected from canine subjects at the age of 3 weeks. Total RNA isolated from these tissues was reverse transcribed to make representative cDNA pools that wer...