Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels

• Published in: Reproduction in domestic animals Vol. 54; no. 1; pp. 31 - 38
• Main Authors: Nascimento, Tiago S., Silva, Isabelle S. M., Alves, Maria Cecília M. A., Gouveia, Bruna B., Barbosa, Lara Mariane R., Macedo, Taís J. S., Santos, Jamile M. S., Monte, Alane P. O., Matos, Maria Helena T., Padilha, Francine F., Lima‐Verde, Isabel B.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.01.2019
• Subjects: Animals; antioxidants; Cell Culture Techniques - veterinary; Culture; Culture Media - chemistry; development; Encapsulation; Female; Flavonoids; Follicles; Glutathione; Glutathione - analysis; High performance liquid chromatography; Liquid chromatography; Mitochondria; Mitochondria - physiology; Nanoparticles; Nanoparticles - administration & dosage; nanotechnology; oocyte; Ovarian Follicle - drug effects; Ovarian Follicle - physiology; ovine; Oxidative Stress; Particle size; Polydispersity; Propolis; Propolis - administration & dosage; Propolis - chemistry; Propolis - pharmacology; Reactive oxygen species; Sheep; Sheep, Domestic; nanotechnology; development; ovine; oocyte; antioxidants

Contents This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high‐performance liquid chromatography (HPLC) and determinatio...