Characterization of protein unable to bind von Willebrand factor in recombinant factor VIII products

• Published in: Journal of thrombosis and haemostasis Vol. 19; no. 4; pp. 954 - 966
• Main Authors: Chun, Haarin, Pettersson, John R., Shestopal, Svetlana A., Wu, Wells W., Marakasova, Ekaterina S., Olivares, Philip, Surov, Stepan S., Ovanesov, Mikhail V., Shen, Rong‐Fong, Sarafanov, Andrey G.
• Format: Journal Article
• Language: English
• Published: England Elsevier Limited 01.04.2021
• Subjects: affinity; Affinity chromatography; Animals; Blood Coagulation Tests; chromatography; Coagulation factor VIII; Coagulation factors; Factor VIII; Gel electrophoresis; Hemophilia; hemophilia A; Hemophilia A - drug therapy; Hemostatics; Immunoblotting; low density lipoprotein receptor‐related protein‐1; Mass spectroscopy; Mice; Protein structure; Proteins; Receptor density; Surface plasmon resonance; Von Willebrand factor; hemophilia A; low density lipoprotein receptor-related protein-1; von Willebrand Factor; factor VIII; chromatography; affinity

Background Therapeutic products with coagulation factor VIII (FVIII) have a wide range of specific activities, implying presence of protein with altered structure. Previous studies showed that recombinant FVIII products (rFVIII) contain a fraction (FVIIIFT) unable to bind von Willebrand factor (VWF)...