DNA adducts in urothelial cells: Relationship with biomarkers of exposure to arylamines and polycyclic aromatic hydrocarbons from tobacco smoke

• Published in: International journal of cancer Vol. 65; no. 3; pp. 314 - 316
• Main Authors: Vineis, Paolo, Talaska, Glenn, Malaveille, Christian, Bartsch, Helmut, Martone, Tiziana, Sithisarankul, Pornchai, Strickland, Paul
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 26.01.1996
• Subjects: Aminobiphenyl Compounds - adverse effects; Cotinine - urine; DNA Adducts - biosynthesis; DNA Adducts - urine; Humans; Male; Middle Aged; Nicotine - urine; Pyrenes - adverse effects; Smoking - adverse effects; Urinary Bladder - cytology; Urinary Bladder - drug effects; Urinary Bladder - metabolism
• DOI: 10.1002/(SICI)1097-0215(19960126)65:3<314::AID-IJC6>3.0.CO;2-2
• ISSN: 0020-7136

Markers of exposure to polycyclic aromatic hydrocarbons (urinary 1‐hydroxypyrene‐glucuronide) and aromatic amines (4‐aminobiphenyl‐hemoglobin adducts), as well as urinary mutagenicity, were measured in 47 healthy smokers and 50 non‐smokers. DNA adducts were determined by P32‐postlabeling in the exfoliated bladder cells of 39 healthy subjects. Both 1‐hydroxypyrene‐glucuronide (1‐OHPG) and 4‐aminobiphenyl adducts (4‐ABP‐Hb) were associated with smoking habits, but only 4‐ABP‐Hb adducts were associated with consumption of black, air‐cured tobacco. The levels of 2 DNA adducts (numbers 2 and 4) in urothelial cells were clearly associated with 4‐ABP‐Hb adducts, in all subjects and in smokers. Levels of one of these DNA adducts (number 2) were also associated with 1‐hydroxypyrene‐glucuronide in urines, but in smokers the association was not statistically significant. Overall, these observations constitute further evidence of a role of arylamines in tobacco‐induced bladder cancer. © 1996 Wiley‐Liss, Inc.