Accelerated vascularization of a novel collagen hydrogel dermal template
Full thickness skin loss is a debilitating problem, most commonly reconstructed using split thickness skin grafts (STSG), which do not reconstitute normal skin thickness and often result in suboptimal functional and esthetic outcomes that diminish a patient's quality of life. To address the min...
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Published in | Journal of tissue engineering and regenerative medicine Vol. 16; no. 12; pp. 1173 - 1183 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Hindawi Limited
01.12.2022
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Abstract | Full thickness skin loss is a debilitating problem, most commonly reconstructed using split thickness skin grafts (STSG), which do not reconstitute normal skin thickness and often result in suboptimal functional and esthetic outcomes that diminish a patient's quality of life. To address the minimal dermis present in most STSG, engineered dermal templates were developed that can induce tissue ingrowth and the formation of neodermal tissue. However, clinically available dermal templates have many shortcomings including a relatively slow rate and degree of neovascularization (∼2–4 weeks), resulting in multiple dressing changes, prolonged immobilization, and susceptibility to infection. Presented herein is a novel composite hydrogel scaffold that optimizes a unique scaffold microarchitecture with native hydrogel properties and mechanical cues ideal for promoting neovascularization, tissue regeneration, and wound healing. In vitro analysis demonstrated the unique combination of improved mechanical attributes with native hydrogel properties that promotes cell invasion and remodeling within the scaffold. In a novel 2‐stage rat model of full thickness skin loss that closely mimics clinical practice, the composite hydrogel induced rapid cell infiltration and neovascularization, creating a healthy neodermis after only 1 week onto which a skin graft could be placed. The scaffold also elicited a gradual and favorable immune response, resulting in more efficient integration into the host. We have developed a dermal scaffold that utilizes simple but unique collagen hydrogel architectural cues that rapidly induces the formation of stable, functional neodermal tissue, which holds tremendous promise for the treatment of full thickness skin loss. |
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AbstractList | Full thickness skin loss is a debilitating problem, most commonly reconstructed using split thickness skin grafts (STSG), which do not reconstitute normal skin thickness and often result in suboptimal functional and esthetic outcomes that diminish a patient's quality of life. To address the minimal dermis present in most STSG, engineered dermal templates were developed that can induce tissue ingrowth and the formation of neodermal tissue. However, clinically available dermal templates have many shortcomings including a relatively slow rate and degree of neovascularization (∼2–4 weeks), resulting in multiple dressing changes, prolonged immobilization, and susceptibility to infection. Presented herein is a novel composite hydrogel scaffold that optimizes a unique scaffold microarchitecture with native hydrogel properties and mechanical cues ideal for promoting neovascularization, tissue regeneration, and wound healing. In vitro analysis demonstrated the unique combination of improved mechanical attributes with native hydrogel properties that promotes cell invasion and remodeling within the scaffold. In a novel 2‐stage rat model of full thickness skin loss that closely mimics clinical practice, the composite hydrogel induced rapid cell infiltration and neovascularization, creating a healthy neodermis after only 1 week onto which a skin graft could be placed. The scaffold also elicited a gradual and favorable immune response, resulting in more efficient integration into the host. We have developed a dermal scaffold that utilizes simple but unique collagen hydrogel architectural cues that rapidly induces the formation of stable, functional neodermal tissue, which holds tremendous promise for the treatment of full thickness skin loss. Full thickness skin loss is a debilitating problem, most commonly reconstructed using split thickness skin grafts (STSG), which do not reconstitute normal skin thickness and often result in suboptimal functional and esthetic outcomes that diminish a patient's quality of life. To address the minimal dermis present in most STSG, engineered dermal templates were developed that can induce tissue ingrowth and the formation of neodermal tissue. However, clinically available dermal templates have many shortcomings including a relatively slow rate and degree of neovascularization (∼2–4 weeks), resulting in multiple dressing changes, prolonged immobilization, and susceptibility to infection. Presented herein is a novel composite hydrogel scaffold that optimizes a unique scaffold microarchitecture with native hydrogel properties and mechanical cues ideal for promoting neovascularization, tissue regeneration, and wound healing. In vitro analysis demonstrated the unique combination of improved mechanical attributes with native hydrogel properties that promotes cell invasion and remodeling within the scaffold. In a novel 2‐stage rat model of full thickness skin loss that closely mimics clinical practice, the composite hydrogel induced rapid cell infiltration and neovascularization, creating a healthy neodermis after only 1 week onto which a skin graft could be placed. The scaffold also elicited a gradual and favorable immune response, resulting in more efficient integration into the host. We have developed a dermal scaffold that utilizes simple but unique collagen hydrogel architectural cues that rapidly induces the formation of stable, functional neodermal tissue, which holds tremendous promise for the treatment of full thickness skin loss. |
Author | Spector, Jason A. Cohen, Rachael Sapir‐Lekhovitser, Yulia Weisel, Adam |
Author_xml | – sequence: 1 givenname: Adam orcidid: 0000-0002-9773-1170 surname: Weisel fullname: Weisel, Adam organization: FesariusTherapeutics, Inc – sequence: 2 givenname: Rachael surname: Cohen fullname: Cohen, Rachael organization: FesariusTherapeutics, Inc – sequence: 3 givenname: Jason A. surname: Spector fullname: Spector, Jason A. organization: Cornell University – sequence: 4 givenname: Yulia surname: Sapir‐Lekhovitser fullname: Sapir‐Lekhovitser, Yulia email: yulia.lekhovitser@fesariustherapeutics.com organization: FesariusTherapeutics, Inc |
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Cites_doi | 10.1073/pnas.86.3.933 10.1098/rsif.2009.0403 10.1007/s00018‐016‐2252‐8 10.1111/wrr.12119 10.1073/pnas.1115973108 10.1159/000454919 10.1089/ten.TEB.2014.0086 10.3390/biom10081169 10.1016/j.biomaterials.2010.07.072 10.1586/erd.11.27 10.1016/j.actbio.2019.04.027 10.3390/ijms17121974 10.2147/CCID.S50046 10.1016/j.jcws.2012.03.001 10.1007/978-3-7091-1586-2 10.1186/s12938‐019‐0647‐0 10.1016/S1369‐7021(08)70087‐7 10.1186/s41038‐016‐0027‐y 10.1007/s11095‐011‐0378‐9 10.1098/rsif.2006.0179 10.1533/9780857091383.2.184 10.1080/23320885.2022.2047052 10.1039/C4TB00614C 10.1097/00000658‐198110000‐00005 10.1063/5.0038364 10.1001/archopthalmol.2011.1178 10.1002/adhm.201500005 10.1073/pnas.48.2.138 |
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References | 2019; 91 2010; 31 2021; 5 2015; 5 1989; 86 2011 2009 2019; 18 2016; 73 2006; 3 2004 2008; 11 2008; 4 2020; 10 2016; 17 2011; 3 2012; 12 2015; 7 2016; 58 2011; 8 2014; 22 2014; 20 2016; 4 2012; 130 1981; 194 2014; 4 2011; 108 2020 2017; 32 2022; 9 1962; 48 2007; 4 2013 2011; 28 2010; 7 2014; 32 e_1_2_10_24_1 e_1_2_10_21_1 e_1_2_10_22_1 e_1_2_10_20_1 Braza M. E. (e_1_2_10_5_1) 2020 Kamran A. (e_1_2_10_18_1) 2012; 12 e_1_2_10_4_1 e_1_2_10_3_1 e_1_2_10_19_1 e_1_2_10_6_1 e_1_2_10_17_1 e_1_2_10_8_1 e_1_2_10_14_1 ISO (e_1_2_10_16_1) 2009 e_1_2_10_37_1 e_1_2_10_7_1 e_1_2_10_36_1 e_1_2_10_12_1 e_1_2_10_35_1 Integra LifeSciences (e_1_2_10_15_1) 2004 e_1_2_10_34_1 e_1_2_10_10_1 e_1_2_10_33_1 e_1_2_10_11_1 e_1_2_10_32_1 e_1_2_10_31_1 e_1_2_10_30_1 Chitturi R. T. (e_1_2_10_9_1) 2015; 7 Furman J. (e_1_2_10_13_1) 2006; 3 Ahearne M. (e_1_2_10_2_1) 2008; 4 e_1_2_10_29_1 Nasalpure A. V. (e_1_2_10_23_1) 2017; 32 e_1_2_10_27_1 e_1_2_10_28_1 e_1_2_10_25_1 e_1_2_10_26_1 |
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Snippet | Full thickness skin loss is a debilitating problem, most commonly reconstructed using split thickness skin grafts (STSG), which do not reconstitute normal skin... |
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SubjectTerms | Animals Collagen Collagen - pharmacology Computer architecture Dermis dermis regeneration full‐thickness skin loss hydrogel composite scaffold Hydrogels Hydrogels - pharmacology Immobilization Immune response Quality of Life Rats Regeneration (physiology) Regenerative medicine Scaffolds Skin Skin grafts Skin Transplantation - methods Thickness Tissue engineering Tissues Vascularization Wound Healing |
Title | Accelerated vascularization of a novel collagen hydrogel dermal template |
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