Serum protein profiling in patients with inflammatory bowel diseases using selective solid-phase bulk extraction, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and chemometric data analysis

• Published in: Rapid communications in mass spectrometry Vol. 21; no. 24; pp. 4142 - 4148
• Main Authors: Nanni, Paolo, Parisi, Daniela, Roda, Giulia, Casale, Monica, Belluzzi, Andrea, Roda, Enrico, Mayer, Lloyd, Roda, Aldo
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 30.12.2007
• Subjects: Adult; Aged; Biomarkers - blood; Blood Proteins - analysis; Blood Proteins - classification; Colitis, Ulcerative - blood; Colitis, Ulcerative - diagnosis; Crohn Disease - blood; Crohn Disease - diagnosis; Discriminant Analysis; Female; Humans; Male; Middle Aged; Peptide Mapping; Predictive Value of Tests; Spectrometry, Mass, Electrospray Ionization - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

The identification of new biomarkers or a disease‐related protein fingerprint for inflammatory bowel diseases (IBDs) represents a major task in the diagnosis, prognosis and pharmacological therapy. To address these issues, a simple and rapid analytical proteomic method for serum protein profiling ba...