Single‐stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein production

• Published in: Biotechnology progress Vol. 39; no. 2; pp. e3323 - n/a
• Main Authors: O'Mara, Brian, Singh, Naveen Kumar, Menendez, Alicia, Tipton, Barbara, Vail, Andrew, Voloshin, Alexei, Buechler, Ying, Anderson, Sean M.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.03.2023; Wiley Subscription Services, Inc
• Subjects: Adsorptivity; Animals; Bioreactors; Cell culture; Centrifugation; Charge coupled devices; CHO Cells; Chromatography; Cricetinae; Cricetulus; depth filtration; fiber chromatography; Filters; Filtration; Filtration - methods; Fluid filters; host cell protein; Impurities; Labor; Lipids; Membrane filters; Nucleic acids; Ovaries; process economics; Proteins; Recombinant Proteins - genetics; Robust control; Utilities; yield; fiber chromatography; host cell protein; depth filtration; process economics; yield
• DOI: 10.1002/btpr.3323
• ISSN: 8756-7938

A single‐stage clarification was developed using a single‐use chromatographic clarification device (CCD) to recover a recombinant protein from Chinese Hamster Ovary (CHO) harvest cell culture fluid (HCCF). Clarification of a CHO HCCF is a complex and costly process, involving multiple stages of centrifugation and/or depth filtration to remove cells and debris and to reduce process‐related impurities such as host cell protein (HCP), nucleic acids, and lipids. When using depth filtration, the filter train consists of multiple filters of varying ratios, layers, pore sizes, and adsorptive properties. The depth filters, in combination with a 0.2‐micron membrane filter, clarify the HCCF based on size‐exclusion, adsorptive, and charge‐based mechanisms, and provide robust bioburden control. Each stage of the clarification process requires time, labor, and utilities, with product loss at each step. Here, use of the 3M™ Harvest RC Chromatographic Clarifier, a single‐stage CCD, is identified as an alternative strategy to a three‐stage filtration train. The CCD results in less overall filter area, less volume for flushing, and higher yield. Using bioprocess cost modeling, the single‐stage clarification process was compared to a three‐stage filtration process. By compressing the CHO HCCF clarification to a single chromatographic stage, the overall cost of the clarification process was reduced by 17%–30%, depending on bioreactor scale. The main drivers for the cost reduction were reduced total filtration area, labor, time, and utilities. The benefits of the single‐stage harvest process extended throughout the downstream process, resulting in a 25% relative increase in cumulative yield with comparable impurity clearance.