Expansion of Fcγ Receptor IIIa–Positive Macrophages, Ficolin 1–Positive Monocyte‐Derived Dendritic Cells, and Plasmacytoid Dendritic Cells Associated With Severe Skin Disease in Systemic Sclerosis

• Published in: Arthritis & rheumatology (Hoboken, N.J.) Vol. 74; no. 2; pp. 329 - 341
• Main Authors: Xue, Dan, Tabib, Tracy, Morse, Christina, Yang, Yi, Domsic, Robyn T., Khanna, Dinesh, Lafyatis, Robert
• Format: Journal Article
• Language: English
• Published: Boston, USA Wiley Periodicals, Inc 01.02.2022; Wiley Subscription Services, Inc
• Subjects: Biopsy; CC chemokine receptors; CCR1 protein; Cell activation; Chemokines; Clustering; Dendritic cells; Dendritic Cells - immunology; Dendritic structure; Embedding; Fibrosis; Ficolins; Gene sequencing; Humans; Immune system; Immunofluorescence; Innate immunity; Lectins - biosynthesis; Lectins - immunology; Lymphocytes B; Macrophages; Macrophages - immunology; Macrophages - metabolism; MARCO protein; Monocytes; Monocytes - immunology; Monocytes - metabolism; Myeloid cells; Pathogenesis; Progenitor cells; Receptors; Receptors, IgG - biosynthesis; Receptors, IgG - immunology; Scleroderma; Scleroderma, Diffuse - immunology; Severity of Illness Index; Skin diseases; Systemic sclerosis; Transcription; Transcriptomes

Objective In this study, we sought a comprehensive understanding of myeloid cell types driving fibrosis in diffuse cutaneous systemic sclerosis (dcSSc) skin. Methods We analyzed the transcriptomes of 2,465 myeloid cells from skin biopsy specimens from 12 dcSSc patients and 10 healthy control subject...