Chromosome replication patterns in the hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus (Methanococcus) jannaschii

• Published in: Molecular microbiology Vol. 45; no. 5; pp. 1443 - 1450
• Main Authors: Maisnier‐Patin, Sophie, Malandrin, Laurence, Birkeland, Nils‐Kåre, Bernander, Rolf
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.09.2002; Blackwell Publishing Ltd
• Subjects: Archaeoglobus fulgidus - cytology; Archaeoglobus fulgidus - genetics; Archaeoglobus fulgidus - growth & development; Base Sequence; Cell Cycle; Chromosomes, Archaeal - genetics; DNA, Archaeal - genetics; Genetic Markers; Genome, Archaeal; Methanococcus - cytology; Methanococcus - genetics; Methanococcus - growth & development; Physical Chromosome Mapping; Replication Origin
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1046/j.1365-2958.2002.03111.x

Summary We analysed chromosome replication patterns in the two hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus (Methanococcus) jannaschii by marker frequency analysis (MFA). For A. fulgidus, the central region of the chromosomal physical map displayed a higher relative abundance in gene dosage during exponential growth, with two continuous gradients to a region of lower abundance at the diametrically opposite side of the genome map. This suggests bidirectional replication of the A. fulgidus chromosome from a single origin. The organization of the putative replication origin region relative to the cdc6, mcm and DNA polymerase genes differed from that reported for Pyrococcus species. No single replication origin or termination regions could be identified for M. jannaschii, adding to the list of unusual properties of this organism. The organization of the A. fulgidus cell cycle was characterized by flow cytometry analysis of the samples from which genomic DNA was extracted for MFA. The relative lengths of the cell cycle periods were found to be similar to those of crenarchaea.