Flow Cytometry: An Overview

• Published in: Current protocols in immunology Vol. 120; p. 5.1.1
• Main Author: McKinnon, Katherine M
• Format: Journal Article
• Language: English
• Published: United States 21.02.2018
• Subjects: Data Analysis; Flow Cytometry - instrumentation; Flow Cytometry - methods; Humans; Indicators and Reagents; flow cytometry; light scatter; fluorescence; reagents

Flow cytometry is a technology that provides rapid multi-parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals that are read by detectors such as photodiodes or photomultiplier tubes. These light signa...