A Diversified Library of Bacterial and Fungal Bifunctional Cytochrome P450 Enzymes for Drug Metabolite Synthesis

• Published in: Advanced synthesis & catalysis Vol. 351; no. 13; pp. 2140 - 2146
• Main Authors: Weis, Roland, Winkler, Margit, Schittmayer, Matthias, Kambourakis, Spiros, Vink, Mandy, Rozzell, J. David, Glieder, Anton
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag 01.09.2009
• Subjects: active pharmaceutical ingredients (APIs); Aspergillus fumigatus; cytochrome P450 (CYP); Escherichia coli; hydroxylation; metabolites; mutagenesis

Innovative biohydroxylation catalysts for the preparation of drug metabolites were developed from scratch. A set of bacterial and fungal sequences of putative and already known bifunctional P450 enzymes was identified by protein sequence alignments, expressed in Escherichia coli and characterised. N...