Neuron-glia cross talk revealed in reverberating networks by simultaneous extracellular recording of spikes and astrocytes' glutamate transporter and K + currents

• Published in: Journal of neurophysiology Vol. 116; no. 6; pp. 2706 - 2719
• Main Authors: Wanke, Enzo, Gullo, Francesca, Dossi, Elena, Valenza, Gaetano, Becchetti, Andrea
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.12.2016
• Series: Glial Cells and Neuronal Signaling
• Subjects: Action Potentials - physiology; Amino Acid Transport System X-AG - metabolism; Animals; Animals, Newborn; Aspartic Acid - pharmacology; beta-Fructofuranosidase - pharmacology; Call for Papers; Cell Communication - drug effects; Cell Communication - physiology; Cells, Cultured; Cerebral Cortex - cytology; Electric Stimulation; Evoked Potentials - drug effects; Evoked Potentials - physiology; In Vitro Techniques; Mice; Nerve Net - physiology; Neuroglia - drug effects; Neuroglia - physiology; Neurons - drug effects; Neurons - physiology; Neurotransmitter Agents - pharmacology; Organ Culture Techniques; Patch-Clamp Techniques; Potassium - metabolism; MEA; neocortex; AraC; K+ buffering; TBOA

Astrocytes uptake synaptically released glutamate with electrogenic transporters (GluT) and buffer the spike-dependent extracellular K + excess with background K + channels. We studied neuronal spikes and the slower astrocytic signals on reverberating neocortical cultures and organotypic slices from...