Suspended aggregates as an immobilization mode for high-density perfusion culture of HEK 293 cells in a stirred tank bioreactor
Cells of the human embryonic kidney cell line (HEK 293) grown in repeated suspension and perfusion systems were characterized and described. Cell aggregates that formed immediately after the HEK 293 cells were inoculated in stirred vessels in serum-containing Dulbecco's modified Eagle's me...
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Published in | Applied microbiology and biotechnology Vol. 72; no. 6; pp. 1144 - 1151 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
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Berlin
Springer
01.10.2006
Springer Nature B.V |
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Abstract | Cells of the human embryonic kidney cell line (HEK 293) grown in repeated suspension and perfusion systems were characterized and described. Cell aggregates that formed immediately after the HEK 293 cells were inoculated in stirred vessels in serum-containing Dulbecco's modified Eagle's medium (D-MEM)/F-12 medium. The mean diameter of the cell aggregates reflecting the aggregate size increased with culture time, shifting from 63 to 239 mum after 1 and 8 days of culture in spinner flasks, respectively. No significant differences in cell performance were observed between HEK 293 cell populations grown as suspended aggregates and those grown as anchored monolayers. Replacing the D-MEM/F-12 with CD 293 medium caused the compact spherical cell aggregates to dissociate into single cells and small irregular aggregates without any apparent effect on cell performance. Moreover, the spherical cell aggregates could reform from individual cells and small aggregates when exposed to the serum-containing D-MEM/F-12 dominant medium. Perfusion culture of HEK 293 cells grown as suspended aggregates in a 7.5-l stirred tank bioreactor for 17 days resulted in a maximum viable cell density of 1.2 x 10(7) cells ml(-1). These results demonstrate the feasibility and proof-of-concept for using aggregates as an immobilization system in large-scale stirred bioreactors because a small-scale culture can be used as easily as the inoculum for larger bioreactors. |
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AbstractList | Cells of the human embryonic kidney cell line (HEK 293) grown in repeated suspension and perfusion systems were characterized and described. Cell aggregates that formed immediately after the HEK 293 cells were inoculated in stirred vessels in serum-containing Dulbecco's modified Eagle's medium (D-MEM)/F-12 medium. The mean diameter of the cell aggregates reflecting the aggregate size increased with culture time, shifting from 63 to 239 μm after 1 and 8 days of culture in spinner flasks, respectively. No significant differences in cell performance were observed between HEK 293 cell populations grown as suspended aggregates and those grown as anchored monolayers. Replacing the D-MEM/F-12 with CD 293 medium caused the compact spherical cell aggregates to dissociate into single cells and small irregular aggregates without any apparent effect on cell performance. Moreover, the spherical cell aggregates could reform from individual cells and small aggregates when exposed to the serum-containing D-MEM/F-12 dominant medium. Perfusion culture of HEK 293 cells grown as suspended aggregates in a 7.5-l stirred tank bioreactor for 17 days resulted in a maximum viable cell density of 1.2×10^sup 7^ cells ml^sup -1^. These results demonstrate the feasibility and proof-of-concept for using aggregates as an immobilization system in large-scale stirred bioreactors because a small-scale culture can be used as easily as the inoculum for larger bioreactors.[PUBLICATION ABSTRACT] Cells of the human embryonic kidney cell line (HEK 293) grown in repeated suspension and perfusion systems were characterized and described. Cell aggregates that formed immediately after the HEK 293 cells were inoculated in stirred vessels in serum-containing Dulbecco's modified Eagle's medium (D-MEM)/F-12 medium. The mean diameter of the cell aggregates reflecting the aggregate size increased with culture time, shifting from 63 to 239 mu m after 1 and 8 days of culture in spinner flasks, respectively. No significant differences in cell performance were observed between HEK 293 cell populations grown as suspended aggregates and those grown as anchored monolayers. Replacing the D-MEM/F-12 with CD 293 medium caused the compact spherical cell aggregates to dissociate into single cells and small irregular aggregates without any apparent effect on cell performance. Moreover, the spherical cell aggregates could reform from individual cells and small aggregates when exposed to the serum-containing D-MEM/F-12 dominant medium. Perfusion culture of HEK 293 cells grown as suspended aggregates in a 7.5-l stirred tank bioreactor for 17 days resulted in a maximum viable cell density of 1.2 x 10 super(7) cells ml super(-1). These results demonstrate the feasibility and proof-of-concept for using aggregates as an immobilization system in large-scale stirred bioreactors because a small-scale culture can be used as easily as the inoculum for larger bioreactors. Cells of the human embryonic kidney cell line (HEK 293) grown in repeated suspension and perfusion systems were characterized and described. Cell aggregates that formed immediately after the HEK 293 cells were inoculated in stirred vessels in serum-containing Dulbecco's modified Eagle's medium (D-MEM)/F-12 medium. The mean diameter of the cell aggregates reflecting the aggregate size increased with culture time, shifting from 63 to 239 mum after 1 and 8 days of culture in spinner flasks, respectively. No significant differences in cell performance were observed between HEK 293 cell populations grown as suspended aggregates and those grown as anchored monolayers. Replacing the D-MEM/F-12 with CD 293 medium caused the compact spherical cell aggregates to dissociate into single cells and small irregular aggregates without any apparent effect on cell performance. Moreover, the spherical cell aggregates could reform from individual cells and small aggregates when exposed to the serum-containing D-MEM/F-12 dominant medium. Perfusion culture of HEK 293 cells grown as suspended aggregates in a 7.5-l stirred tank bioreactor for 17 days resulted in a maximum viable cell density of 1.2 x 10(7) cells ml(-1). These results demonstrate the feasibility and proof-of-concept for using aggregates as an immobilization system in large-scale stirred bioreactors because a small-scale culture can be used as easily as the inoculum for larger bioreactors. |
Author | LIU, H LI, S. C WANG, Q. W CHEN, Z. L LIU, X. M HUANG, P. T WU, B. C YE, L. L |
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Cites_doi | 10.1007/BF02626461 10.1002/bit.260410203 10.1002/bit.20566 10.1021/bp049830x 10.1023/A:1007932614011 10.1002/bit.10629 10.1002/bit.260460408 10.1038/nbt1026 10.1111/j.1749-6632.1994.tb44368.x 10.1002/bit.260410204 10.1016/S1389-1723(00)80052-3 10.1099/0022-1317-36-1-59 10.1385/MB:15:3:249 10.1016/S0958-1669(00)00080-X 10.1007/s00253-005-1980-8 10.1016/j.biomaterials.2004.06.017 10.1016/0141-0229(92)90067-X 10.1007/s00253-002-0991-y 10.1007/BF00365491 10.1023/A:1024992706678 10.1007/BF00754614 10.2144/03345st02 10.1016/S0958-1669(00)00197-X 10.1016/S0734-9750(02)00030-7 10.1023/A:1008040221630 |
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References | JL Moreira (409_CR13) 1995; 46 J Grunberg (409_CR7) 2003; 34 J Jones (409_CR9) 2005; 91 N Wu (409_CR24) 2000; 11 S Yamamoto (409_CR26) 2000; 89 A Kadouri (409_CR10) 1997; 24 I Chevalot (409_CR3) 1994; 16 S Goetgheheur (409_CR6) 1991; 34 D Voisard (409_CR23) 2003; 82 WA Renner (409_CR18) 1993; 41 M Reiter (409_CR17) 1990; 3 FM Wurm (409_CR25) 2004; 22 FL Garham (409_CR5) 1977; 36 L Baldi (409_CR1) 2005; 21 JH Ryu (409_CR19) 2003; 25 P Iyer (409_CR8) 1999; 30 JL Moreira (409_CR12) 1994; 745 L Chu (409_CR4) 2001; 12 MV Peshwa (409_CR16) 1993; 41 M Butler (409_CR2) 2005; 68 MS Sinacore (409_CR21) 2000; 15 JH Ryu (409_CR20) 2005; 26 K Yamada (409_CR27) 1999; 88 I Nadeau (409_CR14) 2003; 20 WR Tolbert (409_CR22) 1980; 16 TJ Nikolai (409_CR15) 1992; 14 G Kretzmer (409_CR11) 2002; 59 |
References_xml | – volume: 16 start-page: 486 year: 1980 ident: 409_CR22 publication-title: In Vitro doi: 10.1007/BF02626461 contributor: fullname: WR Tolbert – volume: 41 start-page: 179 year: 1993 ident: 409_CR16 publication-title: Biotechnol Bioeng doi: 10.1002/bit.260410203 contributor: fullname: MV Peshwa – volume: 91 start-page: 722 issue: 6 year: 2005 ident: 409_CR9 publication-title: Biotechnol Bioeng doi: 10.1002/bit.20566 contributor: fullname: J Jones – volume: 21 start-page: 148 issue: 1 year: 2005 ident: 409_CR1 publication-title: Biotechnol Prog doi: 10.1021/bp049830x contributor: fullname: L Baldi – volume: 88 start-page: 557 issue: 5 year: 1999 ident: 409_CR27 publication-title: J Biotechnol Bioeng contributor: fullname: K Yamada – volume: 24 start-page: 89 year: 1997 ident: 409_CR10 publication-title: Cytotechnology doi: 10.1023/A:1007932614011 contributor: fullname: A Kadouri – volume: 82 start-page: 751 issue: 7 year: 2003 ident: 409_CR23 publication-title: Biotechnol Bioeng doi: 10.1002/bit.10629 contributor: fullname: D Voisard – volume: 46 start-page: 351 year: 1995 ident: 409_CR13 publication-title: Biotechnol Bioeng doi: 10.1002/bit.260460408 contributor: fullname: JL Moreira – volume: 22 start-page: 1393 year: 2004 ident: 409_CR25 publication-title: Nat Biotechnol doi: 10.1038/nbt1026 contributor: fullname: FM Wurm – volume: 745 start-page: 122 year: 1994 ident: 409_CR12 publication-title: Ann N Y Acad Sci doi: 10.1111/j.1749-6632.1994.tb44368.x contributor: fullname: JL Moreira – volume: 41 start-page: 188 year: 1993 ident: 409_CR18 publication-title: Biotechnol Bioeng doi: 10.1002/bit.260410204 contributor: fullname: WA Renner – volume: 89 start-page: 534 year: 2000 ident: 409_CR26 publication-title: J Biosci Bioeng doi: 10.1016/S1389-1723(00)80052-3 contributor: fullname: S Yamamoto – volume: 36 start-page: 59 year: 1977 ident: 409_CR5 publication-title: J Gen Virol doi: 10.1099/0022-1317-36-1-59 contributor: fullname: FL Garham – volume: 15 start-page: 249 year: 2000 ident: 409_CR21 publication-title: Mol Biotechnol doi: 10.1385/MB:15:3:249 contributor: fullname: MS Sinacore – volume: 11 start-page: 205 year: 2000 ident: 409_CR24 publication-title: Curr Opin Biotechnol doi: 10.1016/S0958-1669(00)00080-X contributor: fullname: N Wu – volume: 68 start-page: 283 year: 2005 ident: 409_CR2 publication-title: Appl Microbiol Biotechnol doi: 10.1007/s00253-005-1980-8 contributor: fullname: M Butler – volume: 26 start-page: 2173 year: 2005 ident: 409_CR20 publication-title: Biomaterials doi: 10.1016/j.biomaterials.2004.06.017 contributor: fullname: JH Ryu – volume: 34 start-page: 735 year: 1991 ident: 409_CR6 publication-title: Appl Microbiol Biotechnol contributor: fullname: S Goetgheheur – volume: 14 start-page: 203 year: 1992 ident: 409_CR15 publication-title: Enzyme Microb Technol doi: 10.1016/0141-0229(92)90067-X contributor: fullname: TJ Nikolai – volume: 59 start-page: 135 year: 2002 ident: 409_CR11 publication-title: Appl Microbiol Biotechnol doi: 10.1007/s00253-002-0991-y contributor: fullname: G Kretzmer – volume: 3 start-page: 271 year: 1990 ident: 409_CR17 publication-title: Cytotechnology doi: 10.1007/BF00365491 contributor: fullname: M Reiter – volume: 25 start-page: 1363 year: 2003 ident: 409_CR19 publication-title: Biotechnol Lett doi: 10.1023/A:1024992706678 contributor: fullname: JH Ryu – volume: 16 start-page: 121 year: 1994 ident: 409_CR3 publication-title: Cytotechnology doi: 10.1007/BF00754614 contributor: fullname: I Chevalot – volume: 34 start-page: 968 issue: 5 year: 2003 ident: 409_CR7 publication-title: Biotechniques doi: 10.2144/03345st02 contributor: fullname: J Grunberg – volume: 12 start-page: 180 year: 2001 ident: 409_CR4 publication-title: Curr Opin Biotechnol doi: 10.1016/S0958-1669(00)00197-X contributor: fullname: L Chu – volume: 20 start-page: 475 year: 2003 ident: 409_CR14 publication-title: Biotechnol Adv doi: 10.1016/S0734-9750(02)00030-7 contributor: fullname: I Nadeau – volume: 30 start-page: 169 year: 1999 ident: 409_CR8 publication-title: Cytotechnology doi: 10.1023/A:1008040221630 contributor: fullname: P Iyer |
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Title | Suspended aggregates as an immobilization mode for high-density perfusion culture of HEK 293 cells in a stirred tank bioreactor |
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