Ultra-sensitive detection of pathogenic microorganism using surface-engineered impedimetric immunosensor
A label-free electrochemical immunosensor for highly sensitive and specific detection of pathogenic bacteria is described. To increase the sensitivity of electrochemical impedance spectroscopy (EIS) based immunosensor the surface of gold electrode was engineered with a biocompatible and hydrophilic...
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| Published in | Sensors and actuators. B, Chemical Vol. 161; no. 1; pp. 824 - 831 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
01.01.2012
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| Subjects | |
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| Abstract | A label-free electrochemical immunosensor for highly sensitive and specific detection of pathogenic bacteria is described. To increase the sensitivity of electrochemical impedance spectroscopy (EIS) based immunosensor the surface of gold electrode was engineered with a biocompatible and hydrophilic layer of hyaluronic acid (HA) which greatly enhanced the capture efficiency of model microorganisms,
Escherichia coli O157:H7 through anti-
E. coli O157:H7 antibody conjugated onto the HA layer. For quantitative detection, the magnitude of impedance at 0.1
Hz in the presence of redox probe, [Fe(CN)
6]
3−/4−, was analyzed by normalized impedance change (NIC) with respect to varying concentration of target bacteria. The magnitude of impedance at 0.1
Hz increased linearly with the concentration of target bacteria (10–10
5
cfu/mL) with an extremely low limit of detection (∼7
cfu/mL). This is by far one of the most sensitive non-PCR based sensors for bacterial detection. The responses of this system against non-target bacteria,
Staphylococcus aureus,
Bacillus cereus and non-pathogenic
E. coli DH5α were negligible even at a high concentration, which confirms the highly specific nature of antibody–HA modified immunosensor. |
|---|---|
| AbstractList | A label-free electrochemical immunosensor for highly sensitive and specific detection of pathogenic bacteria is described. To increase the sensitivity of electrochemical impedance spectroscopy (EIS) based immunosensor the surface of gold electrode was engineered with a biocompatible and hydrophilic layer of hyaluronic acid (HA) which greatly enhanced the capture efficiency of model microorganisms,
Escherichia coli O157:H7 through anti-
E. coli O157:H7 antibody conjugated onto the HA layer. For quantitative detection, the magnitude of impedance at 0.1
Hz in the presence of redox probe, [Fe(CN)
6]
3−/4−, was analyzed by normalized impedance change (NIC) with respect to varying concentration of target bacteria. The magnitude of impedance at 0.1
Hz increased linearly with the concentration of target bacteria (10–10
5
cfu/mL) with an extremely low limit of detection (∼7
cfu/mL). This is by far one of the most sensitive non-PCR based sensors for bacterial detection. The responses of this system against non-target bacteria,
Staphylococcus aureus,
Bacillus cereus and non-pathogenic
E. coli DH5α were negligible even at a high concentration, which confirms the highly specific nature of antibody–HA modified immunosensor. A label-free electrochemical immunosensor for highly sensitive and specific detection of pathogenic bacteria is described. To increase the sensitivity of electrochemical impedance spectroscopy (EIS) based immunosensor the surface of gold electrode was engineered with a biocompatible and hydrophilic layer of hyaluronic acid (HA) which greatly enhanced the capture efficiency of model microorganisms, Escherichia coli O157:H7 through anti-E. coli O157:H7 antibody conjugated onto the HA layer. For quantitative detection, the magnitude of impedance at 0.1Hz in the presence of redox probe, [Fe(CN)₆]³⁻/⁴⁻, was analyzed by normalized impedance change (NIC) with respect to varying concentration of target bacteria. The magnitude of impedance at 0.1Hz increased linearly with the concentration of target bacteria (10–10⁵cfu/mL) with an extremely low limit of detection (∼7cfu/mL). This is by far one of the most sensitive non-PCR based sensors for bacterial detection. The responses of this system against non-target bacteria, Staphylococcus aureus, Bacillus cereus and non-pathogenic E. coli DH5α were negligible even at a high concentration, which confirms the highly specific nature of antibody–HA modified immunosensor. |
| Author | Joung, Cho-Kyung Im, Hae-Cheon Kim, Young-Rok Oh, Mi-Hwa Kim, Han-Nah Kim, Hae-Yeong |
| Author_xml | – sequence: 1 givenname: Cho-Kyung surname: Joung fullname: Joung, Cho-Kyung organization: Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea – sequence: 2 givenname: Han-Nah surname: Kim fullname: Kim, Han-Nah organization: Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea – sequence: 3 givenname: Hae-Cheon surname: Im fullname: Im, Hae-Cheon organization: Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea – sequence: 4 givenname: Hae-Yeong surname: Kim fullname: Kim, Hae-Yeong organization: Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea – sequence: 5 givenname: Mi-Hwa surname: Oh fullname: Oh, Mi-Hwa organization: National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea – sequence: 6 givenname: Young-Rok surname: Kim fullname: Kim, Young-Rok email: youngkim@khu.ac.kr organization: Institute of Life Sciences and Resources & Department of Food Science and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 446-701, Republic of Korea |
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| Keywords | Electroanalytical methods Surface modification E. coli O157:H7 Biosensors Hyaluronic acid |
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| Snippet | A label-free electrochemical immunosensor for highly sensitive and specific detection of pathogenic bacteria is described. To increase the sensitivity of... |
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| SubjectTerms | Bacillus cereus bacteria Biosensors detection limit dielectric spectroscopy E. coli O157:H7 Electroanalytical methods electrochemistry electrodes Escherichia coli O157 gold Hyaluronic acid hydrophilicity immunosensors impedance iron microbial detection Staphylococcus aureus Surface modification |
| Title | Ultra-sensitive detection of pathogenic microorganism using surface-engineered impedimetric immunosensor |
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