Effect of Ca2+ Ions on the Adhesion and Mechanical Properties of Adsorbed Layers of Human Osteopontin

Using an atomic force microscope and a surface force apparatus, we measured the surface coverage, adhesion, and mechanical properties of layers of osteopontin (OPN), a phosphoprotein of the human bones, adsorbed on mica. OPN is believed to connect mineralized collagen fibrils of the bone in a matrix...

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Published inBiophysical journal Vol. 95; no. 6; pp. 2939 - 2950
Main Authors Zappone, Bruno, Thurner, Philipp J., Adams, Jonathan, Fantner, Georg E., Hansma, Paul K.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.09.2008
The Biophysical Society
Subjects
Adsorption - drug effects
Aluminum Silicates - metabolism
Biomechanical Phenomena
Buffers
Calcium - pharmacology
Humans
Microscopy, Atomic Force
Osteopontin - chemistry
Osteopontin - metabolism
Proteins
Sodium Hydroxide - chemistry
Surface Properties
Time Factors
Online AccessGet full text

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Abstract Using an atomic force microscope and a surface force apparatus, we measured the surface coverage, adhesion, and mechanical properties of layers of osteopontin (OPN), a phosphoprotein of the human bones, adsorbed on mica. OPN is believed to connect mineralized collagen fibrils of the bone in a matrix that dissipates energy, reducing the risk of fractures. Atomic force microscopy normal force measurements showed large adhesion and energy dissipation upon retraction of the tip, which were due to the breaking of the many OPN-OPN and OPN-mica bonds formed during tip-sample contact. The dissipated energy increased in the presence of Ca2+ ions due to the formation of additional OPN-OPN and OPN-mica salt bridges between negative charges. The forces measured by surface force apparatus between two macroscopic mica surfaces were mainly repulsive and became hysteretic only in the presence of Ca2+: adsorbed layers underwent an irreversible compaction during compression due to the formation of long-lived calcium salt bridges. This provides an energy storage mechanism, which is complementary to energy dissipation and may be equally relevant to bone recovery after yield. The prevalence of one mechanism or the other appears to depend on the confinement geometry, adsorption protocol, and loading-unloading rates.
AbstractList Using an atomic force microscope and a surface force apparatus, we measured the surface coverage, adhesion, and mechanical properties of layers of osteopontin (OPN), a phosphoprotein of the human bones, adsorbed on mica. OPN is believed to connect mineralized collagen fibrils of the bone in a matrix that dissipates energy, reducing the risk of fractures. Atomic force microscopy normal force measurements showed large adhesion and energy dissipation upon retraction of the tip, which were due to the breaking of the many OPN-OPN and OPN-mica bonds formed during tip-sample contact. The dissipated energy increased in the presence of Ca(2+) ions due to the formation of additional OPN-OPN and OPN-mica salt bridges between negative charges. The forces measured by surface force apparatus between two macroscopic mica surfaces were mainly repulsive and became hysteretic only in the presence of Ca(2+): adsorbed layers underwent an irreversible compaction during compression due to the formation of long-lived calcium salt bridges. This provides an energy storage mechanism, which is complementary to energy dissipation and may be equally relevant to bone recovery after yield. The prevalence of one mechanism or the other appears to depend on the confinement geometry, adsorption protocol, and loading-unloading rates.
Using an atomic force microscope and a surface force apparatus, we measured the surface coverage, adhesion, and mechanical properties of layers of osteopontin (OPN), a phosphoprotein of the human bones, adsorbed on mica. OPN is believed to connect mineralized collagen fibrils of the bone in a matrix that dissipates energy, reducing the risk of fractures. Atomic force microscopy normal force measurements showed large adhesion and energy dissipation upon retraction of the tip, which were due to the breaking of the many OPN-OPN and OPN-mica bonds formed during tip-sample contact. The dissipated energy increased in the presence of Ca2+ ions due to the formation of additional OPN-OPN and OPN-mica salt bridges between negative charges. The forces measured by surface force apparatus between two macroscopic mica surfaces were mainly repulsive and became hysteretic only in the presence of Ca2+: adsorbed layers underwent an irreversible compaction during compression due to the formation of long-lived calcium salt bridges. This provides an energy storage mechanism, which is complementary to energy dissipation and may be equally relevant to bone recovery after yield. The prevalence of one mechanism or the other appears to depend on the confinement geometry, adsorption protocol, and loading-unloading rates.
Using an atomic force microscope and a surface force apparatus, we measured the surface coverage, adhesion, and mechanical properties of layers of osteopontin (OPN), a phosphoprotein of the human bones, adsorbed on mica. OPN is believed to connect mineralized collagen fibrils of the bone in a matrix that dissipates energy, reducing the risk of fractures. Atomic force microscopy normal force measurements showed large adhesion and energy dissipation upon retraction of the tip, which were due to the breaking of the many OPN-OPN and OPN-mica bonds formed during tip-sample contact. The dissipated energy increased in the presence of Ca 2+ ions due to the formation of additional OPN-OPN and OPN-mica salt bridges between negative charges. The forces measured by surface force apparatus between two macroscopic mica surfaces were mainly repulsive and became hysteretic only in the presence of Ca 2+ : adsorbed layers underwent an irreversible compaction during compression due to the formation of long-lived calcium salt bridges. This provides an energy storage mechanism, which is complementary to energy dissipation and may be equally relevant to bone recovery after yield. The prevalence of one mechanism or the other appears to depend on the confinement geometry, adsorption protocol, and loading-unloading rates.
Author Adams, Jonathan
Zappone, Bruno
Hansma, Paul K.
Thurner, Philipp J.
Fantner, Georg E.
AuthorAffiliation Liquid Crystal Laboratory, Regional Laboratory and Center of Excellence for Functional Nanostructured Materials, Centro Nazionale delle Ricerche and Istituto Nazionale per la Fisica della Materia, Arcavacata di Rende (CS) 87036, Italy; † Bioengineering Science Research Group, University of Southampton, Southampton, United Kingdom; ‡ Department of Physics, University of California, Santa Barbara, California; and § Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts
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Notes Address reprint requests to Bruno Zappone, E-mail: zappone@fis.unical.it.
Editor: Jane Clarke.
Bruno Zappone and Philipp J. Thurner contributed equally to this work.
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SSID ssj0012501
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Snippet Using an atomic force microscope and a surface force apparatus, we measured the surface coverage, adhesion, and mechanical properties of layers of osteopontin...
SourceID pubmedcentral
crossref
pubmed
elsevier
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 2939
SubjectTerms Adsorption - drug effects
Aluminum Silicates - metabolism
Biomechanical Phenomena
Buffers
Calcium - pharmacology
Humans
Microscopy, Atomic Force
Osteopontin - chemistry
Osteopontin - metabolism
Proteins
Sodium Hydroxide - chemistry
Surface Properties
Time Factors
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Title Effect of Ca2+ Ions on the Adhesion and Mechanical Properties of Adsorbed Layers of Human Osteopontin
URI https://dx.doi.org/10.1529/biophysj.108.135889
https://www.ncbi.nlm.nih.gov/pubmed/18586839
https://pubmed.ncbi.nlm.nih.gov/PMC2527241
Volume 95
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