Engineering of a fungal laccase to develop a robust, versatile and highly-expressed biocatalyst for sustainable chemistry

• Published in: Green chemistry : an international journal and green chemistry resource : GC Vol. 21; no. 19; pp. 5374 - 5385
• Main Authors: de Salas, Felipe, Aza, Pablo, Gilabert, Joan F, Santiago, Gerard, Kilic, Sibel, Sener, Mehmet E, Vind, Jesper, Guallar, Víctor, Martínez, Angel T, Camarero, Susana
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 30.09.2019
• Subjects: Aniline; anionic surfactants; Aspergillus oryzae; Biocatalysts; Chemical synthesis; Computer applications; Computer simulation; Directed evolution; dyeing; dyes; Enzymes; fabrics; Fungi; Green chemistry; High temperature; Laccase; Organic chemistry; Oxidation; pH effects; polyaniline; Polyanilines; Polymerization; Redox potential; Saccharomyces cerevisiae; Secretion; Stability; Substrates; synthesis; temperature

Fungal laccases can play an important role as biocatalysts in organic chemistry to replace chemical synthesis. In a previous work we synthesized conductive polyaniline using a high-redox potential laccase from our collection of recombinant fungal variants. Still, the oxidation of aniline is hindered...