Development of Hydrogel Microparticle based RT-qPCR for Advanced Detection of BCR-ABL1 Transcripts

• Published in: Biochip journal Vol. 13; no. 2; pp. 182 - 190
• Main Authors: Kim, Jung Min, Kim, Won Jin, Kim, Mi Yeon, Kim, Kwang Pyo, Sim, Sang Jun, Kim, Sang Kyung
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.06.2019; Springer Nature B.V; 한국바이오칩학회
• Subjects: Binding sites; Biomedical Engineering and Bioengineering; Biotechnology; Cancer; Chemistry; Design; Efficiency; Genes; Hybridization; Hydrogels; Kinases; Microparticles; Mutation; Original Article; Polymerase chain reaction; Reverse transcription; Ribonucleic acid; RNA; Target detection; 생물공학; United States > US; TaqMan probe; N Real-time PCR; Chronic myeloid leukemia; Hydrogel microparticle; RT-qPCR; BCR-ABL

Reverse transcription – quantitative polymerase chain reaction (RT-qPCR) is conventionally used method to analyze oncogenes, infected DNAs, and mutated tumor associated genes. However it is hard to detect rare genetic targets since the disturbance of undesired amplification often overrides the react...