Production of stable isotope labelled lipase Lip2 from Yarrowia lipolytica for NMR: Investigation of several expression systems

• Published in: Protein expression and purification Vol. 101; pp. 14 - 20
• Main Authors: Nars, G., Saurel, O., Bordes, F., Saves, I., Remaud-Siméon, M., André, I., Milon, A., Marty, A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2014; Elsevier
• Subjects: Catalytic Domain; Cell-free; Cell-Free System - metabolism; Chemical and Process Engineering; Crystallography, X-Ray; Engineering Sciences; Escherichia coli - genetics; Escherichia coli - metabolism; Food engineering; Fungal Proteins - biosynthesis; Fungal Proteins - chemistry; Fungal Proteins - genetics; Gene Expression - genetics; Isotope Labeling - methods; Isotope labelling; Life Sciences; Lipase - biosynthesis; Lipase - chemistry; Lipase - genetics; Nuclear Magnetic Resonance, Biomolecular; Pichia - genetics; Pichia - metabolism; Pichia pastoris; Yarrowia - enzymology; Yarrowia - genetics; Yarrowia - metabolism; Yarrowia lipolytica; Yarrowia lipolytica lipase lip2; Yeast; Yarrowia lipolytica lipase lip2; Cell-free; Yeast; Pichia pastoris; Isotope labelling; Yarrowia lipolytica; HETEROLOGOUS PROTEIN EXPRESSION; CELLS; QUANTITIES; ENANTIOSELECTIVITY; YEAST PICHIA-PASTORIS; RECOMBINANT; PURIFICATION; DYNAMICS; BINDING; ACID-ESTERS

•Yarrowia lipolytica, Pichia pastoris, Escherichia coli and cell free expression were tested.•Yarrowia lipolytica was the most efficient system for production of 15N labelled Lip2.•First 15N–1H HSQC spectra of Lip2 was recorded. Extracellular lipase Lip2 from Yarrowia lipolytica is a promising bioca...