Production of stable isotope labelled lipase Lip2 from Yarrowia lipolytica for NMR: Investigation of several expression systems - Nars, G.; Saurel, O.; Bordes, F.; Saves, I.; Remaud-Siméon, M.; André, I.; Milon, A.; Marty, A. (Protein expression and purification, 2014)
Keywords: Catalytic Domain; Cell-free; Cell-Free System - metabolism; Chemical and Process Engineering; Crystallography, X-Ray; Engineering Sciences; Escherichia coli - genetics; Escherichia coli - metabolism; Food engineering; Fungal Proteins - biosynthesis; Fungal Proteins - chemistry; Fungal Proteins - genetics; Gene Expression - genetics; Isotope Labeling - methods; Isotope labelling; Life Sciences; Lipase - biosynthesis; Lipase - chemistry; Lipase - genetics; Nuclear Magnetic Resonance, Biomolecular; Pichia - genetics; Pichia - metabolism; Pichia pastoris; Yarrowia - enzymology; Yarrowia - genetics; Yarrowia - metabolism; Yarrowia lipolytica; Yarrowia lipolytica lipase lip2; Yeast; Yarrowia lipolytica lipase lip2; Cell-free; Yeast; Pichia pastoris; Isotope labelling; Yarrowia lipolytica; HETEROLOGOUS PROTEIN EXPRESSION; CELLS; QUANTITIES; ENANTIOSELECTIVITY; YEAST PICHIA-PASTORIS; RECOMBINANT; PURIFICATION; DYNAMICS; BINDING; ACID-ESTERS