Detection and Evaluation of Non-Recombinants in cDNA Libraries by Multiple Cloning Region PCR

• Published in: BioTechniques Vol. 32; no. 1; pp. 88 - 92
• Main Authors: Bandyopadhyay, Dilip, Vesuna, Farhad D
• Format: Journal Article
• Language: English
• Published: Natick, MA Future Science Ltd 01.01.2002; Eaton; Taylor & Francis Group
• Subjects: Base Sequence; Biological and medical sciences; Biotechnology; Cloning, Molecular - methods; DNA Primers; DNA, Complementary; Evaluation Studies as Topic; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; In vitro gene amplification. Pcr technique; Methods. Procedures. Technologies; Molecular and cellular biology; Plasmids; Polymerase Chain Reaction - methods; Recombination, Genetic; Evaluation; Multiple; Variable; Selection; Biology; Virus; Polymerase chain reaction; Complementary DNA; Proportion; Recombinant protein; Molecular cloning; Detection; Species; Phage

Bacteriophages that are routinely used in cDNA libraries do not require any biological selection for forming plaques. Thus parental non-recombinant phages are always found in variable proportions together with recombinant ones in all cDNA libraries. The presence of non-recombinants in significant pr...