Epitope prediction and confirmation for the human androgen receptor: Generation of monoclonal antibodies for multi-assay performance following the synthetic peptide strategy

• Published in: Biochimica et biophysica acta Vol. 1073; no. 1; pp. 23 - 32
• Main Authors: Zegers, Netty D., Claassen, Eric, Neelen, Conny, Mulder, Eppo, van Laar, Jacoba H., Voorhorst, Margreet M., Berrevoets, Cor A., Brinkmann, Albert O., van der Kwast, Theodorus H., Ruizeveld de Winter, Jacobus A., Trapman, Jan, Boersma, Wim J.A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.01.1991; Elsevier
• Subjects: Amino Acid Sequence; Androgen receptor; Antibodies, immunoglobulins; Antibodies, Monoclonal - immunology; Antibody; Antigen-Antibody Complex - chemistry; Biological and medical sciences; Diagnostic; Epitopes; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Human; Humans; Immunohistochemistry; MAb performance; Male; Molecular immunology; Molecular Sequence Data; Monoclonal antibodies; Peptides - chemical synthesis; Peptides - immunology; Prostate - immunology; Protein Conformation; Receptors, Androgen - chemistry; Receptors, Androgen - immunology; Structure-Activity Relationship; Synthetic peptide; Immunohistochemistry; Human; Antibody; KLH; R1881; Diagnostic; MAb; PAb; MAb performance; BSA; Androgen receptor; hAR; GA; Synthetic peptide; SP; ELISA; Synthetic product; Antigenic determinant; Peptides; Androgen hormone; Monoclonal antibody; Diagnosis; Histochemistry; Immunological method; Hormonal receptor

The human androgen receptor (hAR) is an important regulatory protein particularly in male sexual differentiation. The investigation of hAR functionality has been hampered by the lack of AR specific monoclonal antibodies recognizing the functional domains of the receptor. Therefore production of high...