Use of narrow-bore high-performance liquid chromatography for microanalysis of protein structure

• Published in: Journal of chromatography Vol. 368; no. 2; p. 215
• Main Authors: Lu, H S, Lai, P H
• Format: Journal Article
• Language: English
• Published: Netherlands 1986
• Subjects: Amino Acid Sequence; Amino Acids - analysis; Chromatography, High Pressure Liquid; Humans; Hydrolysis; Isothiocyanates; Kallikreins - urine; Microchemistry; Peptides - isolation & purification; Protein Conformation; Proteins - isolation & purification; Thiocyanates

We report here systematic approaches to microanalysis of protein structures using narrow-bone high-performance liquid chromatography of protein, peptide and derivatized [phenylthiocarbamyl (PTC-) and phenylthiohydantoinyl (PTH-)] amino acids. The utilization of columns with small diameters (2 mm or...