Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic β2-microglobulin variant

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 4; pp. 1314 - 1319
• Main Authors: Domanska, Katarzyna, Vanderhaegen, Saskia, Srinivasan, Vasundara, Pardon, Els, Dupeux, Florine, Marquez, Jose A, Giorgetti, Sofia, Stoppini, Monica, Wyns, Lode, Bellotti, Vittorio, Steyaert, Jan
• Format: Journal Article
• Language: English
• Published: National Academy of Sciences 25.01.2011; National Acad Sciences
• Subjects: Aggregation; Amyloids; Antibodies; Biological Sciences; Cell aggregates; Crystal structure; Dimers; Monomers; Oligomers; Proteins; Solar fibrils

Atomic-level structural investigation of the key conformational intermediates of amyloidogenesis remains a challenge. Here we demonstrate the utility of nanobodies to trap and characterize intermediates of β2-microglobulin (β2m) amyloidogenesis by X-ray crystallography. For this purpose, we selected...