12alpha-Hydroxysteroid dehydrogenase from Clostridium group P strain C48-50 ATCC No. 29733: partial purification and characterization

• Published in: Journal of lipid research Vol. 20; no. 2; pp. 234 - 239
• Main Authors: Macdonald, I A, Jellett, J F, Mahony, D E
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.1979; Elsevier
• Subjects: Binding Sites; Cholic Acids; Clostridium - enzymology; Hydroxysteroid Dehydrogenases - isolation & purification; Hydroxysteroid Dehydrogenases - metabolism; Kinetics; Oxidation-Reduction; Substrate Specificity

The growth of Clostridium group P strain C48-50 [an anaerobe that contains 12alpha-hydroxysteroid dehydrogenase (12alpha-HSDH) in the absence of other dehydrogenases active upon bile salts] is greatly enhanced by the addition of 2.0% d-fructose or d-glucose to the growth medium. Other sugars were le...