Apple fruit copper amine oxidase isoforms: peroxisomal MdAO1 prefers diamines as substrates, whereas extracellular MdAO2 exclusively utilizes monoamines

• Published in: Plant and cell physiology Vol. 56; no. 1; pp. 137 - 147
• Main Authors: Zarei, Adel, Trobacher, Christopher P, Cooke, Alison R, Meyers, Ashley J, Hall, J Christopher, Shelp, Barry J
• Format: Journal Article
• Language: English
• Published: Japan 01.01.2015
• Subjects: Allium cepa; Amine Oxidase (Copper-Containing) - genetics; Amine Oxidase (Copper-Containing) - metabolism; Amino Acid Sequence; Arabidopsis; Arabidopsis - cytology; Arabidopsis - enzymology; Arabidopsis - genetics; Biogenic Monoamines - metabolism; Biosynthetic Pathways; Diamines - metabolism; Extracellular Space - enzymology; Fruit - cytology; Fruit - enzymology; Fruit - genetics; gamma-Aminobutyric Acid - metabolism; Gene Expression Regulation, Plant; Isoenzymes; Malus - enzymology; Malus - genetics; Malus domestica; Molecular Sequence Data; Onions - cytology; Onions - enzymology; Onions - genetics; Organ Specificity; Oxidation-Reduction; Peroxisomes - enzymology; Plant Proteins - genetics; Plant Proteins - metabolism; Polyamines - metabolism; Sequence Alignment; Substrate Specificity; Recombinant expression; Abiotic stress; 4-Aminobutyrate; Subcellular localization; Copper amine oxidase; Apple fruit; Enzyme kinetics; Polyamines

4-Aminobutyrate (GABA) accumulates in apple fruit during controlled atmosphere storage. A potential source of GABA is the polyamine putrescine, which can be oxidized via copper-containing amine oxidase (CuAO), resulting in the production 4-aminobutanal/Δ(1)-pyrroline, with the consumption of O2 and...